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C-Erbb2, P185 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603153

c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor… More details

$1155 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
c-erbB2, p185, Monoclonal Antibody
Full Product Name
c-erbB2, p185 (HER2, neu Receptor)
Product Synonym Names
Anti -c-erbB2, p185 (HER2, neu Receptor)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.N.211
Host
Mouse
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide. See C0015-20X.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes c-erbB-2 at 185kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues 1242-1255 (TAENPEYLGLDVPV) from the C-terminus of human c-erbB-2 protein, KLH-conjugated.
Cellular Localization
Cell membrane
Positive Control
SKBR-3 or MAD109 cells, breast carcinomas.

Product Categories/Family for anti-c-erbB2, p185 antibody

Antibodies; Abs to Oncoproteins
Applications Tested/Suitable for anti-c-erbB2, p185 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-c-erbB2, p185 antibody
Suitable for use in Immunoprecipitation, Western Blot, Immunohistology., , Recommended Dilution:, Immunoprecipitation: 2ug antibody/mg protein lysate (Use Protein G)., Western Blot: 0.5-1.0ug/ml for 2 hours at RT., Immunohistology: 0.5-1ug/ml for 30 minutes at RT., Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0., Optimal dilutions to be determined by the researcher.

Testing Data of anti-c-erbB2, p185 antibody

Mouse Anti-C-Erbb2, P185 Monoclonal Antibody – Cat# MBS603153

Testing Data of anti-c-erbB2, p185 antibody

Mouse Anti-C-Erbb2, P185 Monoclonal Antibody, Testing Data – Cat# MBS603153

Testing Data of anti-c-erbB2, p185 antibody

Mouse Anti-C-Erbb2, P185 Monoclonal Antibody, Testing Data – Cat# MBS603153

Other Notes

Small volumes of anti-c-erbB2, p185 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-erbB2, p185 purchase

MBS603153 is an antibody targeting c-erbB2, p185. Also known as c-erbB2, p185 (HER2, neu Receptor). Reactive in Human, Monkey, Mouse, Rat. Host: Mouse.
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