Mouse Anti-NOS2 Monoclonal Antibody – Cat# MBS603220

Nitrotyrosine (NOS2) Antibody – Mouse Monoclonal

Name: NOS2 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS603220
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603220

The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety… More details

$830 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Nitrotyrosine (NOS2), Monoclonal Antibody

Full Product Name

Nitrotyrosine (nTyr)

Product Gene Name

anti-NOS2 antibody

Product Synonym Names

Anti -Nitrotyrosine (nTyr)

Clonality

Monoclonal

Isotype

IgG1,k

Clone Number

6D609

Host

Mouse

Species Reactivity

Canine, Human, Mouse, Rat

Form/Format

Supplied as a lyophilized powder from PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute in 1ml sterile ddH2O.

Purity/Purification

Purified
Purified by thiophilic adsorption and size exclusion chromatography.

Specificity

Recognizes Nitrotyrosine (nTyr). Species Crossreactivity: human, mouse, rat and canine

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

131,208 Da

Chromosome Location

Chromosome: 19; AC_000176.1 (19758764..19790464, complement). Location: chromosome: 19

3D Structure

ModBase 3D Structure for Q27995

Immunogen

KLH modified with nitrotyrosine

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine

Applications Tested/Suitable for anti-NOS2 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-NOS2 antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.05ug/ml
Western Blot: 1ug/ml for AP/BCIP/NBT (MTT detection) or 0.1ug/ml for HRPO/ECL detection.

Other Notes

Small volumes of anti-NOS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOS2 purchase

MBS603220 is an antibody targeting NOS2. Also known as Nitrotyrosine (nTyr). Reactive in Canine, Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NOS2. It may not necessarily be applicable to this product.

NCBI GI #

116003827

NCBI GeneID

282876

NCBI Accession #

NP_001070267.1

UniProt Primary Accession #

Q27995

UniProt Secondary Accession #

Q06Q84; Q27985

UniProt Related Accession #

Q27995

GenBank Nucleotide #

NM_001076799.1

NCBI Official Full Name

nitric oxide synthase, inducible

NCBI Official Synonym Full Names

nitric oxide synthase 2, inducible

NCBI Official Symbol

NOS2

NCBI Official Synonym Symbols

iNOS; NOS2A

UniProt Protein Name

Nitric oxide synthase, inducible

UniProt Synonym Protein Names

Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2

Protein Family

Nitric oxide synthase

UniProt Gene Name

NOS2

UniProt Synonym Gene Names

Inducible NOS; iNOS; NOSII

UniProt Entry Name

NOS2_BOVIN

NCBI Protein Information

nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)

UniProt Comments for NOS2

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

Research Articles on NOS2

1. Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NOS2 antibody

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram

Diseases associated with anti-NOS2 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2258 publications with NOS2 and Inflammation
Necrosis Antibodies	>1897 publications with NOS2 and Necrosis
Sepsis Antibodies	>543 publications with NOS2 and Sepsis
Intestinal Diseases Antibodies	>381 publications with NOS2 and Intestinal Diseases
Liver Diseases Antibodies	>357 publications with NOS2 and Liver Diseases
Reperfusion Injury Antibodies	>351 publications with NOS2 and Reperfusion Injury
Hypertension Antibodies	>283 publications with NOS2 and Hypertension
Endotoxemia Antibodies	>248 publications with NOS2 and Endotoxemia
Shock, Septic Antibodies	>236 publications with NOS2 and Shock, Septic
Kidney Diseases Antibodies	>214 publications with NOS2 and Kidney Diseases

Organs/Tissues associated with anti-NOS2 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>1149 publications with NOS2 and Muscle
Intestine Antibodies	>459 publications with NOS2 and Intestine
Ovary Antibodies	>47 publications with NOS2 and Ovary
Extraembryonic Tissue Antibodies	>5 publications with NOS2 and Extraembryonic Tissue
Uncharacterized Tissue Antibodies	>1 publications with NOS2 and Uncharacterized Tissue