Mouse Anti-MPS1 Monoclonal Antibody – Cat# MBS603259

HeLa total cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS603259 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system. Arrow indicates Mps1 (~97kD).

MPS1 Antibody – Mouse Monoclonal

Name: MPS1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS603259
Price: 1015 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603259

Mps-1 is a protein kinase thought to play during the spindle checkpoint to regulation of sister chromatids separation when chromsome alingment is complete. More details

$1015 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Mps1, Monoclonal Antibody

Full Product Name

Mps1, CT

Product Gene Name

anti-MPS1 antibody

Product Synonym Names

Anti -Mps1, CT

Clonality

Monoclonal

Isotype

IgG1

Clone Number

5H9

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Mps1, Mr ~97kD. This antibody is known to inhibit the autophosphorylation/ kinase activity of Mps1. Does not react with mouse.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

86,772 Da

3D Structure

ModBase 3D Structure for P54199

Immunogen

His-tagged fusion protein corresponding to aa3-856 of human Mps1, previously known at TTK/PYK.

Product Categories/Family for anti-MPS1 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-MPS1 antibody

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Application Notes for anti-MPS1 antibody

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 0.5-2ug/ml of this lot detected Mps1 in HeLa total cell extract. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with M4685-80 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate Mps1 from HeLa total cell extract.
Immunocytochemistry: Reported by an independent laboratory to show positive immunostaining for Mps1 in U2OS or HeLa cells permeabilized with 0.5% Triton-X-100 and fixed with methanol or paraformaldehyde.

Other Notes

Small volumes of anti-MPS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MPS1 purchase

MBS603259 is an antibody targeting MPS1. Also known as Mps1, CT. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MPS1. It may not necessarily be applicable to this product.

NCBI GI #

1431004

UniProt Primary Accession #

P54199

UniProt Secondary Accession #

D6VRW4

NCBI Official Full Name

MPS1

UniProt Protein Name

Serine/threonine-protein kinase MPS1

UniProt Synonym Protein Names

Monopolar spindle protein 1; Regulatory cell proliferation kinase 1

Protein Family

Probable serine/threonine-protein kinase

UniProt Gene Name

MPS1

UniProt Synonym Gene Names

RPK1

UniProt Entry Name

MPS1_YEAST

UniProt Comments for MPS1

Function: Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Implicated in spindle pole body (SPD) duplication. Dual specificity kinase that can phosphorylate serine, threonine and tyrosine residues. Phosphorylates the SPC29 and SPC110 spindle pole body components. Ref.6 Ref.7

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Post-translational modification: Autophosphorylated.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MPS1 antibody

Reference Product	PubMed Publications
FLNA antibody	>12 publications with MPS1 and FLNA

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