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Chitotriosidase Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603316

Human CHIT-1 (chitotriosidase-1; also chitinase-1) is a 50kD, secreted glycoprotein that belongs to glycosylhydrolase family #18. It is synthesized by macrophages, and may serve to degrade chitin (poly-N-acetylglucosamine)-containing pathogens such as fungi… More details

$1055 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Chitotriosidase, Monoclonal Antibody
Full Product Name
Chitotriosidase (Chitinase 1, CHIT-1)
Product Synonym Names
Anti -Chitotriosidase (Chitinase 1, CHIT-1)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H206
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhCHI-3L1. Species sequence homology
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived rhCHIT-1
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CHIT1.
Applications Tested/Suitable for anti-Chitotriosidase antibody
ELISA (EL/EIA), Immunoprecipitation (IP), Neutralization
Application Notes for anti-Chitotriosidase antibody
Dilution: ELISA (direct): 0.5-1.0ug/ml. Detection limit for rhCHIT1 is approximately 2ng/well.
Immunoprecipitation: 25ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium.
Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N,N',N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56ng/mL (IC 50) under conditions in which the enzyme was present at 20ng/mL and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.

Other Notes

Small volumes of anti-Chitotriosidase antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Chitotriosidase purchase

MBS603316 is an antibody targeting Chitotriosidase. Also known as Chitotriosidase (Chitinase 1, CHIT-1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Chitotriosidase. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
chitotriosidase
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Chitotriosidase antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Chitotriosidase and FLNA
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