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jak1 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603325

Janus Kinase 1 (JAK1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of JAK1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023,… More details

$1095 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
JAK1, Monoclonal Antibody
Full Product Name
JAK1 (Janus Kinase 1)
Product Gene Name
anti-jak1 antibody
Product Synonym Names
Anti -JAK1 (Janus Kinase 1)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7H242
Host
Rat
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human JAK1 at ~130kD. Species Crossreactivity: rat and mouse
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Molecular Weight
132,481 Da
3D Structure
ModBase 3D Structure for O12990
Immunogen
E. coli-derived recombinant human JAK1 (aa20-274, Accession #AAA36527)

Product Categories/Family for anti-jak1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-jak1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-jak1 antibody
Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Flow Cytometry: 25ug/ml; 10ul labels 1-5 x 10e5 Jurkat cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 2% paraformaldehyde and ice-cold methanol. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome.
Immunocytochemistry: 10ug/ml detects JAK1 in HeLa cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted J0900-06A followed by Rhodamine Red-coupled anti-rat IgG in the dark. Between each step, cells were washed with PBS, BSA, Triton X-100.
Immunohistochemistry: 25ug/ml detects JAK1 in paraffin-embedded normal human skin tissue sections.

Testing Data of anti-jak1 antibody

Jurkat cells were stained with MBS603325 (filled histogram) or isotype control antibody (open histogram), followed by PE-conjugated anti-rat antibody.
Rat Anti-jak1 Monoclonal Antibody – Cat# MBS603325

Other Notes

Small volumes of anti-jak1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for jak1 purchase

MBS603325 is an antibody targeting jak1. Also known as JAK1 (Janus Kinase 1). Reactive in Human, Mouse, Rat. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for jak1. It may not necessarily be applicable to this product.

NCBI GI #
226237498
NCBI GeneID
30280
UniProt Primary Accession #
O12990
UniProt Secondary Accession #
O73880
UniProt Related Accession #
O12990; Q7ZU16
NCBI Official Full Name
jak1
NCBI Official Synonym Full Names
Janus kinase 1
NCBI Official Symbol
jak1
NCBI Official Synonym Symbols
jak1
UniProt Protein Name
Tyrosine-protein kinase JAK1
UniProt Synonym Protein Names
Janus kinase 1
Protein Family
Tyrosine-protein kinase
UniProt Gene Name
jak1
UniProt Entry Name
JAK1_DANRE
NCBI Protein Information
tyrosine-protein kinase JAK1; jak-1; jak1 kinase; etID309794.6

UniProt Comments for jak1

Function: Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location: Endomembrane system; Peripheral membrane protein

By similarity. Note: Wholly intracellular, possibly membrane associated

Developmental stage: Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1

Domain: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.
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Research Articles on jak1

1. Required for cell migration of anterior mesendoderm during gastrulation, resulting in extension of the anterior body axis. MO/dominant negative expression gives identical morphotype/phenotype to STAT3alpha knockdown.
2. Required for cell migration of anterior mesendoderm during gastrulation, resulting in extension of the anterior body axis. MO/dominant negative expression gives identical morphotype/phenotype to STAT3alpha knockdown.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-jak1 antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Alpha/beta Signaling Pathway antibodies  Interferon Alpha/beta Signaling Pathway Diagram
 Interleukin Receptor SHC Signaling Pathway antibodies  Interleukin Receptor SHC Signaling Pathway Diagram
 Interleukin-2 Signaling Pathway antibodies  Interleukin-2 Signaling Pathway Diagram

Organs/Tissues associated with anti-jak1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >65 publications with jak1 and Brain
 Muscle Antibodies  >62 publications with jak1 and Muscle
 Heart Antibodies  >53 publications with jak1 and Heart
 Kidney Antibodies  >25 publications with jak1 and Kidney
 Eye Antibodies  >9 publications with jak1 and Eye
 Reproductive System Antibodies  >5 publications with jak1 and Reproductive System
 Whole Body Antibodies  >1 publications with jak1 and Whole Body
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