Mouse Anti-GOLIM4 Monoclonal Antibody – Cat# MBS603394

Figure: Immunocytochemistry usingMBS603394 GPP130 at a 1:1000 dilution.

GOLIM4 Antibody – Mouse Monoclonal

Name: GOLIM4 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS603394
Price: 755 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603394

The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain and mediates trafficking in the late endosome-bypass pathway. GPP130 continuously recycles between early Golgi and distal… More details

$755 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GPP130 (GOLIM4), Monoclonal Antibody

Full Product Name

GPP130 (Golgi Phosphoprotein of 130kD, GIMPc, cis-Golgi Integral Membrane Protein)

Product Gene Name

anti-GOLIM4 antibody

Product Synonym Names

Anti -GPP130 (Golgi Phosphoprotein of 130kD, GIMPc, cis-Golgi Integral Membrane Protein)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

6D892

Host

Mouse

Species Reactivity

Human, Monkey

Form/Format

Lyophilized.

Purity/Purification

Affinity Purified
Affinity purified (HYDRA column) and gel filtrated (FPLC).

Specificity

Recognizes monkey GPP130. Cross-reacts with human GPP130.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

81,880 Da

Chromosome Location

Chromosome: 3; NC_000003.11 (167726457..167813712, complement). Location: 3q26.2

OMIM

606805

3D Structure

ModBase 3D Structure for O00461

Immunogen

GPP130 from Vero monkey cells.

Product Categories/Family for anti-GOLIM4 antibody

Antibodies; Abs to Golgi Proteins

Applications Tested/Suitable for anti-GOLIM4 antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Application Notes for anti-GOLIM4 antibody

Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:1000.
Immunohistochemistry: Frozen sections.
Immunocytochemistry: 1:1000.
Immunoprecipitation: 2ul

Other Notes

Small volumes of anti-GOLIM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOLIM4 purchase

MBS603394 is an antibody targeting GOLIM4. Also known as GPP130 (Golgi Phosphoprotein of 130kD, GIMPc, cis-Golgi Integral Membrane Protein). Reactive in Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GOLIM4. It may not necessarily be applicable to this product.

NCBI GI #

7657138

NCBI GeneID

27333

NCBI Accession #

NP_055313.1

UniProt Primary Accession #

O00461

UniProt Related Accession #

O00461

GenBank Nucleotide #

NM_014498.3

NCBI Official Full Name

Golgi integral membrane protein 4

NCBI Official Synonym Full Names

golgi integral membrane protein 4

NCBI Official Symbol

GOLIM4

NCBI Official Synonym Symbols

P138; GIMPC; GOLPH4; GPP130

UniProt Protein Name

Golgi integral membrane protein 4

UniProt Synonym Protein Names

Golgi integral membrane protein, cis; GIMPc; Golgi phosphoprotein 4; Golgi-localized phosphoprotein of 130 kDa

Protein Family

Golgi integral membrane protein

UniProt Gene Name

GOLIM4

UniProt Synonym Gene Names

GIMPC; GOLPH4; GPP130; GIMPc

UniProt Entry Name

GOLI4_HUMAN

NCBI Protein Information

Golgi integral membrane protein 4; golgi phosphoprotein 4; type II Golgi membrane protein; golgi phosphoprotein of 130 kDa; golgi integral membrane protein, cis; 130 kDa golgi-localized phosphoprotein; golgi-localized phosphoprotein of 130 kDa; cis Golgi-localized calcium-binding protein

NCBI Summary for GOLIM4

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus. [provided by RefSeq, Jul 2008]

UniProt Comments for GOLIM4

GOLPH4: Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi. Belongs to the GOLIM4 family.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 3q26.2

Cellular Component: nucleoplasm; Golgi apparatus; cis-Golgi network; membrane; endocytic vesicle; Golgi lumen; integral to membrane; endosome membrane

Biological Process: transport

Research Articles on GOLIM4

1. the stem domain of GPP130 is a novel Mn sensor in the Golgi lumen of mammalian cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.