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Vasodilator Stimulated Phosphoprotein (VASP) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603408

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF/NO/cGMP Protein kinase and cAMP/cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in… More details

$1225 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Vasodilator Stimulated Phosphoprotein (VASP), Monoclonal Antibody
Full Product Name
Vasodilator Stimulated Phosphoprotein, phosphorylated (VASP) (Ser239)
Product Gene Name
anti-VASP antibody
Product Synonym Names
Anti -Vasodilator Stimulated Phosphoprotein, phosphorylated (VASP) (Ser239)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4i67
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a lyophilized powder from PBS, 0.1% sodium azide, PEG and sucrose. Reconstitute with 1ml sterile ddH2O.
Purity/Purification
Affinity Purified
Puri ed by thiophilic adsorption and size exclusion chromatography.
Specificity
Recognizes human phosphorylated VASP, Mr 50kD.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Molecular Weight
39,830 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (46010688..46030241). Location: 19q13.2-q13.3
OMIM
601703
3D Structure
ModBase 3D Structure for P50552
Immunogen
Synthetic phosphopeptide corresponding to aa236-242 from human phospho-VASP (RKV[pS]KQE) conjugated to KLH.

Product Categories/Family for anti-VASP antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-VASP antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-VASP antibody
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry and Immunoprecipitation.
Dilution: ELISA: 0.05ug/ml, as reported by an independent laboratory.
Western Blot: 0.5-2ug/ml detected phosphorylated VASP in RIPA lysates from CTLL cells. CTLL cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2118 (0.5ug/ml). Proteins were visualized using a Gt xMo IgG (HRP)* and a chemiluminescence detection system.
Immunocytochemistry: An independent laboratory has reported that 3ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, xed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
Flow Cytometry: 2.4ug/ml using xed and permeabilized human platelets, as reported by an independent laboratory.
Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory.

Western Blot (WB) of anti-VASP antibody

Western Blot analysis in CTLL cell lysate probed with MBS603408 (0.5ug/ml) .
Mouse Anti-VASP Monoclonal Antibody – Cat# MBS603408

Other Notes

Small volumes of anti-VASP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VASP purchase

MBS603408 is an antibody targeting VASP. Also known as Vasodilator Stimulated Phosphoprotein, phosphorylated (VASP) (Ser239). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VASP. It may not necessarily be applicable to this product.

NCBI GI #
4507869
NCBI GeneID
7408
NCBI Accession #
NP_003361.1
UniProt Primary Accession #
P50552
UniProt Secondary Accession #
Q6PIZ1; Q93035; B2RBT9
UniProt Related Accession #
P50552
GenBank Nucleotide #
NM_003370.3
NCBI Official Full Name
vasodilator-stimulated phosphoprotein
NCBI Official Synonym Full Names
vasodilator-stimulated phosphoprotein
NCBI Official Symbol
VASP
NCBI Official Synonym Symbols
VASP
UniProt Protein Name
Vasodilator-stimulated phosphoprotein
Protein Family
Protein
UniProt Gene Name
VASP
UniProt Entry Name
VASP_HUMAN
NCBI Protein Information
vasodilator-stimulated phosphoprotein

NCBI Summary for VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]
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UniProt Comments for VASP

VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding

Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization
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Research Articles on VASP

1. Data show that VASP has different immunostaining patterns between cerebral cortical plates in prenatal and adult human brain samples, and suggest that VASP may play a crucial role in the regulation of human neonatal cerebral cortical development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VASP antibody

 Reference Product  PubMed Publications
 ZYX antibody  >28 publications with VASP and ZYX
 PFN1 antibody  >24 publications with VASP and PFN1
 FYB antibody  >7 publications with VASP and FYB
 LPP antibody  >6 publications with VASP and LPP
 PFN2 antibody  >5 publications with VASP and PFN2
 TES antibody  >5 publications with VASP and TES
 ABI1 antibody  >3 publications with VASP and ABI1

Pathways associated with anti-VASP antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-extracellular Matrix Interactions Pathway antibodies  Cell-extracellular Matrix Interactions Pathway Diagram
 E-cadherin Signaling In Keratinocytes Pathway antibodies  E-cadherin Signaling In Keratinocytes Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-VASP antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >94 publications with VASP and Cardiovascular Diseases
 Myocardial Ischemia Antibodies  >63 publications with VASP and Myocardial Ischemia
 Thrombosis Antibodies  >39 publications with VASP and Thrombosis
 Coronary Disease Antibodies  >39 publications with VASP and Coronary Disease
 Pain Antibodies  >31 publications with VASP and Pain
 Acute Coronary Syndrome Antibodies  >24 publications with VASP and Acute Coronary Syndrome
 Myocardial Infarction Antibodies  >19 publications with VASP and Myocardial Infarction
 Inflammation Antibodies  >17 publications with VASP and Inflammation
 Arteriosclerosis Antibodies  >16 publications with VASP and Arteriosclerosis
 Metabolic Diseases Antibodies  >15 publications with VASP and Metabolic Diseases

Organs/Tissues associated with anti-VASP antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >248 publications with VASP and Blood
 Vascular Antibodies  >119 publications with VASP and Vascular
 Muscle Antibodies  >64 publications with VASP and Muscle
 Brain Antibodies  >53 publications with VASP and Brain
 Embryonic Tissue Antibodies  >31 publications with VASP and Embryonic Tissue
 Kidney Antibodies  >24 publications with VASP and Kidney
 Lung Antibodies  >20 publications with VASP and Lung
 Liver Antibodies  >11 publications with VASP and Liver
 Bone Antibodies  >10 publications with VASP and Bone
 Spleen Antibodies  >7 publications with VASP and Spleen
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