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Nestin (NES) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603422

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor… More details

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Product Overview

Online Datasheet in PDF
Product Name
Nestin, Monoclonal Antibody
Full Product Name
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Product Gene Name
anti-NES antibody
Product Synonym Names
Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
9A19
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human nestin. It does not crossreact with rodent protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
177,439 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (156638555..156647189, complement). Location: 1q23.1
OMIM
600915
3D Structure
ModBase 3D Structure for P48681
Immunogen
A fragment of the human nestin protein corresponding to residues 1464-1614.

Product Categories/Family for anti-NES antibody

Antibodies; Abs to Neurofilament
Applications Tested/Suitable for anti-NES antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NES antibody
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Westerm Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.
Immnohistochemistry: Paraffin and Frozen

Western Blot (WB) of anti-NES antibody

Western Blot using MBS603422 (5ug)|Lane 1: Rat's total brain tissue|Lane 2: human CNS progenitor cells (1:1000)
Mouse Anti-NES Monoclonal Antibody – Cat# MBS603422

Immunofluorescence (IF) of anti-NES antibody

Immunocytochemistry/Immunofluorescence using MBS603422 (1:50) staining analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma). The primary antibody was detected using Alexa Fluor 488 labeled IgG (H+L) donkey anti-mouse cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the secondary antibody.
Mouse Anti-NES Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS603422

Immunohistochemistry (IHC) of anti-NES antibody

Immunohistochemistry-Paraffin using MBS603422 (1:500). Nestin was detected in immersion fixed paraffin-embedded sections of human kidney. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).
Mouse Anti-NES Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS603422

Flow Cytometry (FC/FACS) of anti-NES antibody

Flow Cytometry using MBS603422 (1 ug/1x10e6 cells) Intracellular flow cytometric staining of 1 x 10e6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange.
Mouse Anti-NES Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS603422

Other Notes

Small volumes of anti-NES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NES purchase

MBS603422 is an antibody targeting NES. Also known as Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NES. It may not necessarily be applicable to this product.

NCBI GI #
38176300
NCBI GeneID
10763
NCBI Accession #
NP_006608.1
UniProt Primary Accession #
P48681
UniProt Secondary Accession #
O00552; Q3LIF5; Q5SYZ6
UniProt Related Accession #
P48681; Q05BW3; Q2YDX4; Q9H6U9
GenBank Nucleotide #
NM_006617.1
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
FLJ21841; Nbla00170
UniProt Protein Name
Nestin
Protein Family
Nestin
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
NCBI Protein Information
nestin; OTTHUMP00000032198

NCBI Summary for NES

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
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UniProt Comments for NES

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; negative regulation of neuron apoptosis; cell projection morphogenesis; brain development; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
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Research Articles on NES

1. Expression of embryonic stem cell markers SOX2 and nestin in dermatofibrosarcoma protuberans and dermatofibroma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NES antibody

 Reference Product  PubMed Publications
 CDK1 antibody  >3 publications with NES and CDK1

Diseases associated with anti-NES antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >178 publications with NES and Neoplasms
 Cardiovascular Diseases Antibodies  >61 publications with NES and Cardiovascular Diseases
 Vascular Diseases Antibodies  >45 publications with NES and Vascular Diseases
 Leukemia Antibodies  >42 publications with NES and Leukemia
 Congenital Abnormalities Antibodies  >36 publications with NES and Congenital Abnormalities
 Breast Neoplasms Antibodies  >36 publications with NES and Breast Neoplasms
 Neurodegenerative Diseases Antibodies  >29 publications with NES and Neurodegenerative Diseases
 Pain Antibodies  >29 publications with NES and Pain
 Lung Diseases Antibodies  >24 publications with NES and Lung Diseases
 Adenocarcinoma Antibodies  >20 publications with NES and Adenocarcinoma

Organs/Tissues associated with anti-NES antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >151 publications with NES and Brain
 Nerve Antibodies  >91 publications with NES and Nerve
 Muscle Antibodies  >59 publications with NES and Muscle
 Heart Antibodies  >51 publications with NES and Heart
 Eye Antibodies  >38 publications with NES and Eye
 Vascular Antibodies  >36 publications with NES and Vascular
 Kidney Antibodies  >34 publications with NES and Kidney
 Embryonic Tissue Antibodies  >33 publications with NES and Embryonic Tissue
 Lung Antibodies  >26 publications with NES and Lung
 Skin Antibodies  >24 publications with NES and Skin
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