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Myelin Associated Glycoprotein (MAG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603423

Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100kD, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two… More details

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Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Myelin Associated Glycoprotein (MAG), Monoclonal Antibody
Full Product Name
Myelin Associated Glycoprotein (MAG)
Product Gene Name
anti-MAG antibody
Product Synonym Names
Anti -Myelin Associated Glycoprotein (MAG)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q144
Host
Mouse
Species Reactivity
Bovine, Chicken, Frog, Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.5. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes chicken Myelin Associated Glycoprotein at ~100kD. Species Crossreactivity: human, rat, mouse, bovine and frog.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Molecular Weight
69,069 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (35783038..35804707). Location: 19q13.1
OMIM
159460
3D Structure
ModBase 3D Structure for P20916
Immunogen
L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain.
Positive Control
Rat hippocampus tissue, rat brain lysate, PC12 cells

Product Categories/Family for anti-MAG antibody

Antibodies; Abs to Myelin
Applications Tested/Suitable for anti-MAG antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-MAG antibody
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Useful for myelinogenesis and multiple sclerosis studies.
Dilution: Western Blot: 20ug/ml, non-reducing conditions only
Immunohistochemistry: Frozen and Paraffin
Immunocytochemistry: 5-10ug/ml, methanol fixation required. 1. Oligodendrocytes are cultivated on slides and fixed with methanol for 10 minutes at-20 degree C in the middle log-phase.
2. After blocking the nonspecific binding sites with 5% FCS the cells are incubated with the properly diluted M9757-50 in a humid chamber for one hour at RT.
3. Afterwards cells are washed and incubated with anti-Mouse IgG-FITC. The slides are washed and covered appropriately

Testing Data of anti-MAG antibody

Localization of MAG in rat hippocampus using MBS603423.
Mouse Anti-MAG Monoclonal Antibody – Cat# MBS603423

Other Notes

Small volumes of anti-MAG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAG purchase

MBS603423 is an antibody targeting MAG. Also known as Myelin Associated Glycoprotein (MAG). Reactive in Bovine, Chicken, Frog, Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAG. It may not necessarily be applicable to this product.

NCBI GI #
11225258
NCBI GeneID
4099
NCBI Accession #
NP_002352.1
UniProt Primary Accession #
P20916
UniProt Related Accession #
P20916; Q15489; Q53ES7; Q53HA1; Q53HD1; Q567S4; Q59GD9
GenBank Nucleotide #
NM_002361.3
NCBI Official Full Name
myelin-associated glycoprotein isoform a
NCBI Official Synonym Full Names
myelin associated glycoprotein
NCBI Official Symbol
MAG
NCBI Official Synonym Symbols
GMA; S-MAG; SIGLEC4A; SIGLEC-4A
UniProt Protein Name
Myelin-associated glycoprotein
UniProt Synonym Protein Names
Siglec-4a
Protein Family
Allergen
UniProt Gene Name
MAG
UniProt Synonym Gene Names
GMA
UniProt Entry Name
MAG_HUMAN
NCBI Protein Information
myelin-associated glycoprotein; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A

NCBI Summary for MAG

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]
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UniProt Comments for MAG

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: paranode region of axon; plasma membrane; integral to membrane

Molecular Function: carbohydrate binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; blood coagulation; cell adhesion; leukocyte migration
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Research Articles on MAG

1. polysialylated NCAM persistence, up-regulated polysialyltransferase-1 mRNA and previously uncovered defective myelin-associated glycoprotein may be early pathogenetic events in adult-onset autosomal-dominant leukodystrophy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAG antibody

 Reference Product  PubMed Publications
 MBP antibody  >163 publications with MAG and MBP
 RTN4R antibody  >30 publications with MAG and RTN4R
 PLP1 antibody  >14 publications with MAG and PLP1
 FYN antibody  >13 publications with MAG and FYN
 NGFR antibody  >3 publications with MAG and NGFR
 MAP1B antibody  >3 publications with MAG and MAP1B

Pathways associated with anti-MAG antibody

 Products by Pathway  Pathway Diagram
 Axonal Growth Inhibition (RHOA Activation) Pathway antibodies  Axonal Growth Inhibition (RHOA Activation) Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Signalling By NGF Pathway antibodies  Signalling By NGF Pathway Diagram
 P75 NTR Receptor-mediated Signalling Pathway antibodies  P75 NTR Receptor-mediated Signalling Pathway Diagram
 P75(NTR)-mediated Signaling Pathway antibodies  P75(NTR)-mediated Signaling Pathway Diagram
 P75NTR Regulates Axonogenesis Pathway antibodies  P75NTR Regulates Axonogenesis Pathway Diagram

Diseases associated with anti-MAG antibody

 Disease Name  Pubmed Publications
 Demyelinating Diseases Antibodies  >228 publications with MAG and Demyelinating Diseases
 Neoplasms Antibodies  >172 publications with MAG and Neoplasms
 Central Nervous System Diseases Antibodies  >168 publications with MAG and Central Nervous System Diseases
 Female Urogenital Diseases Antibodies  >114 publications with MAG and Female Urogenital Diseases
 Neurologic Manifestations Antibodies  >61 publications with MAG and Neurologic Manifestations
 Mental Disorders Antibodies  >59 publications with MAG and Mental Disorders
 Neurodegenerative Diseases Antibodies  >48 publications with MAG and Neurodegenerative Diseases
 Hypertension Antibodies  >41 publications with MAG and Hypertension
 Inflammation Antibodies  >38 publications with MAG and Inflammation
 Spinal Cord Injuries Antibodies  >37 publications with MAG and Spinal Cord Injuries

Organs/Tissues associated with anti-MAG antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >481 publications with MAG and Nerve
 Brain Antibodies  >417 publications with MAG and Brain
 Blood Antibodies  >385 publications with MAG and Blood
 Kidney Antibodies  >153 publications with MAG and Kidney
 Ganglia Antibodies  >85 publications with MAG and Ganglia
 Spleen Antibodies  >13 publications with MAG and Spleen
 Ovary Antibodies  >9 publications with MAG and Ovary
 Uterus Antibodies  >7 publications with MAG and Uterus
 Mammary Gland Antibodies  >2 publications with MAG and Mammary Gland
 Testis Antibodies  >2 publications with MAG and Testis
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