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Hemagglutinin Tag Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603526

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived… More details

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Sample Manual Insert View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Hemagglutinin Tag, Monoclonal Antibody
Full Product Name
Hemagglutinin Tag (YPYDVPDYA) (HA Tag)
Product Synonym Names
Anti -Hemagglutinin Tag (YPYDVPDYA) (HA Tag)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4i9
Host
Mouse
Form/Format
Supplied in 10mM sodium HEPES, pH 7.5,150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
Purity/Purification
Ascites
Ascites.
Specificity
Detects HA-tagged (YPYDVPDYA) proteins overexpressed in cells. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species Crossreactivity: All.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide containing the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) protein (KLH coupled).
Applications Tested/Suitable for anti-Hemagglutinin Tag antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Hemagglutinin Tag antibody
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunocytochemistry (IF): 1:100
Flow Cytometry: 1:50
Immunohistochemistry: 1:1200

Western Blot (WB) of anti-Hemagglutinin Tag antibody

Western Blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using MBS603526.
Mouse Anti-Hemagglutinin Tag Monoclonal Antibody – Cat# MBS603526

Flow Cytometry (FC/FACS) of anti-Hemagglutinin Tag antibody

Flow Cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Akt3 (green), using MBS603526 compared to a nonspecific negative control antibody (red).
Mouse Anti-Hemagglutinin Tag Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS603526

Immunohistochemistry (IHC) of anti-Hemagglutinin Tag antibody

Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or MBS603526 (bottom).
Mouse Anti-Hemagglutinin Tag Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS603526

Immunofluorescence (IF) of anti-Hemagglutinin Tag antibody

Confocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using MBS603526. Blue pseudocolor = DRAQ5 (Fluorescent DNA dye).
Mouse Anti-Hemagglutinin Tag Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS603526

Western Blot (WB) of anti-Hemagglutinin Tag antibody

Immunoprecipitation of extracts from untransfected COS cells (lanes 2 and 5) or COS cells expressing HA-tagged Akt3 (lane 3) or HA-tagged Estrogen Receptor (ER) (lane 6), using MBS603526 followed by Western Blot analysis using Akt (Rac PKa, PKBa) Pab Rb xMo Catalog No: A1124-03 (lanes 1-3) or Estrogen Receptor, alpha Mab Mo xHu Catalog No: E3565-10A (lanes 4-6).
Mouse Anti-Hemagglutinin Tag Monoclonal Antibody, Western Blot (WB) – Cat# MBS603526

Other Notes

Small volumes of anti-Hemagglutinin Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hemagglutinin Tag purchase

MBS603526 is an antibody targeting Hemagglutinin Tag. Also known as Hemagglutinin Tag (YPYDVPDYA) (HA Tag). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Hemagglutinin Tag. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
hemagglutinin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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