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Upstream Transcription Factor-2 (USF2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603533

MBS603533 is an antibody targeting USF2. Also known as Upstream Transcription Factor-2 (USF2). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Upstream Transcription Factor-2 (USF2), Monoclonal Antibody
Full Product Name
Upstream Transcription Factor-2 (USF2)
Product Gene Name
anti-USF2 antibody
Product Synonym Names
Anti -Upstream Transcription Factor-2 (USF2)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C351
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Upstream Transcription Factor-2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
36,955 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (35759896..35770718). Location: 19q13
OMIM
600390
3D Structure
ModBase 3D Structure for Q15853
Immunogen
Partial sequence of recombinant full-length protein to human Upstream Transcription Factor-2

Product Categories/Family for anti-USF2 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-USF2 antibody
Western Blot (WB)
Application Notes for anti-USF2 antibody
Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000

Testing Data of anti-USF2 antibody

Mouse Anti-USF2 Monoclonal Antibody – Cat# MBS603533

Western Blot (WB) of anti-USF2 antibody

Detection of USF2 by Western Blot. Samples: Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 36 kD
Mouse Anti-USF2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS603533

Immunostaining (IS) of anti-USF2 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-USF2 mAb.
Mouse Anti-USF2 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS603533

Other Notes

Small volumes of anti-USF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USF2. It may not necessarily be applicable to this product.

NCBI GI #
29612623
NCBI GeneID
7392
UniProt Primary Accession #
Q15853
UniProt Secondary Accession #
O00671; O00709; Q05750; Q07952; Q15851; Q15852; Q6FI33
UniProt Related Accession #
Q15853; Q6YI47
NCBI Official Full Name
Upstream transcription factor 2, c-fos interacting
NCBI Official Synonym Full Names
upstream transcription factor 2, c-fos interacting
NCBI Official Symbol
USF2
NCBI Official Synonym Symbols
FIP; bHLHb12
UniProt Protein Name
Upstream stimulatory factor 2
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2
Protein Family
Upstream stimulatory factor
UniProt Gene Name
USF2
UniProt Synonym Gene Names
BHLHB12
UniProt Entry Name
USF2_HUMAN
NCBI Protein Information
upstream stimulatory factor 2; FOS-interacting protein; c-fos interacting protein; major late transcription factor 2; class B basic helix-loop-helix protein 12

NCBI Summary for USF2

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
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UniProt Comments for USF2

USF2: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; lactation; regulation of transcription from RNA polymerase II promoter by glucose; lipid homeostasis; late viral mRNA transcription; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by glucose
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Research Articles on USF2

1. data established that high glucose stimulated USF2 expression in HK-2 cells, at least in part, through angiotensin II-AT1-dependent activation of CREB, which can contribute to diabetic tubulointerstitial fibrosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USF2 antibody

 Reference Product  PubMed Publications
 USF1 antibody  >150 publications with USF2 and USF1
 FOS antibody  >7 publications with USF2 and FOS
 MAF antibody  >2 publications with USF2 and MAF
 EP300 antibody  >2 publications with USF2 and EP300
 IGF2R antibody  >1 publications with USF2 and IGF2R
 BRCA1 antibody  >1 publications with USF2 and BRCA1

Pathways associated with anti-USF2 antibody

 Products by Pathway  Pathway Diagram
 Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway antibodies  Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway Diagram

Diseases associated with anti-USF2 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >7 publications with USF2 and Kidney Diseases
 Breast Neoplasms Antibodies  >6 publications with USF2 and Breast Neoplasms
 Skin Diseases Antibodies  >5 publications with USF2 and Skin Diseases
 Lung Diseases Antibodies  >4 publications with USF2 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with USF2 and Cell Transformation, Neoplastic
 Prostatic Neoplasms Antibodies  >3 publications with USF2 and Prostatic Neoplasms
 Adenoma Antibodies  >2 publications with USF2 and Adenoma
 Lung Neoplasms Antibodies  >2 publications with USF2 and Lung Neoplasms
 Death Antibodies  >2 publications with USF2 and Death
 Chromosome Aberrations Antibodies  >2 publications with USF2 and Chromosome Aberrations

Organs/Tissues associated with anti-USF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >33 publications with USF2 and Liver
 Blood Antibodies  >19 publications with USF2 and Blood
 Kidney Antibodies  >14 publications with USF2 and Kidney
 Brain Antibodies  >11 publications with USF2 and Brain
 Lung Antibodies  >9 publications with USF2 and Lung
 Heart Antibodies  >8 publications with USF2 and Heart
 Ovary Antibodies  >8 publications with USF2 and Ovary
 Pancreas Antibodies  >7 publications with USF2 and Pancreas
 Prostate Antibodies  >4 publications with USF2 and Prostate
 Intestine Antibodies  >4 publications with USF2 and Intestine
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