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RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603609

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD, Monoclonal Antibody
Full Product Name
BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170)
Product Gene Name
Product Synonym Names
Anti -BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C46
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
206,887 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (93683736..93790082). Location: 10q22-q23
OMIM
605191
3D Structure
ModBase 3D Structure for O14981
Immunogen
Partial sequence of recombinant full-length protein to human BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD
Applications Tested/Suitable for anti-BTAF1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-BTAF1 antibody
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2-50ug/ml

Testing Data of anti-BTAF1 antibody

Mouse Anti-BTAF1 Monoclonal Antibody – Cat# MBS603609

Western Blot (WB) of anti-BTAF1 antibody

WB: Detection of BTAF1 by Western Blot. Samples: Whole cell lysate from human HeLa (H, 50ug), mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells. Predicted molecular weight: 206kD.
Mouse Anti-BTAF1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS603609

Testing Data of anti-BTAF1 antibody

FC: HeLA cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS603609 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG.
Mouse Anti-BTAF1 Monoclonal Antibody, Testing Data – Cat# MBS603609

Immunoprecipitation (IP) of anti-BTAF1 antibody

IP: RIPA lysate of HeLa cells was incubated with MBS603609.
Mouse Anti-BTAF1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS603609

Immunostaining (IS) of anti-BTAF1 antibody

IC: Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS603609.
Mouse Anti-BTAF1 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS603609

Other Notes

Small volumes of anti-BTAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTAF1 purchase

MBS603609 is an antibody targeting BTAF1. Also known as BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BTAF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), isoform CRA_a
NCBI Official Synonym Full Names
BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)
NCBI Official Symbol
BTAF1
NCBI Official Synonym Symbols
MOT1; TAF172; KIAA0940; TAFII170; MGC138406; TAF(II)170
UniProt Protein Name
TATA-binding protein-associated factor 172
UniProt Synonym Protein Names
ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; TAF(II)170; TBP-associated factor 172
UniProt Gene Name
BTAF1
UniProt Synonym Gene Names
TAF172
UniProt Entry Name
BTAF1_HUMAN
NCBI Protein Information
TATA-binding protein-associated factor 172; OTTHUMP00000020093; TBP-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit

UniProt Comments for BTAF1

TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family.

Protein type: Transcription initiation complex; EC 3.6.1.-; EC 3.6.4.-; Helicase

Chromosomal Location of Human Ortholog: 10q22-q23

Cellular Component: nucleus

Molecular Function: DNA binding; helicase activity; transcription factor activity; ATP binding

Biological Process: metabolic process; negative regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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