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Rb2, P130 (RBL2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603691

Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105) Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the "pocket… More details

$800 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Rb2, p130 (RBL2), Monoclonal Antibody
Full Product Name
Rb2, p130 (BSA & Azide Free)
Product Gene Name
anti-RBL2 antibody
Product Synonym Names
Anti -Rb2, p130 (BSA & Azide Free)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F327
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See R1200-50
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Reacts only with the un(der) phosphorylated form of Rb2/p130 protein. Species Crossreactivity: human and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
130kD
Immunogen Sequence Positions
878-913
Chromosome Location
Chromosome: 16; NC_000016.9 (53468351..53525560). Location: 16q12.2
OMIM
180203
3D Structure
ModBase 3D Structure for Q08999
Immunogen
A synthetic peptide from p130 corresponding to aa878-913.
Cellular Localization
Nuclear
Positive Control
A427 cells. Tonsil.

Product Categories/Family for anti-RBL2 antibody

Antibodies; Abs to Disease Markers
Applications Tested/Suitable for anti-RBL2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RBL2 antibody
Dilution: Immunofluorescence
Immunoprecipitation (Denatured verified) (Use Protein G; Ab 2ug/mg protein lysate)
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Western Blot (WB) of anti-RBL2 antibody

Mouse Anti-RBL2 Monoclonal Antibody – Cat# MBS603691

Immunohistochemistry (IHC) of anti-RBL2 antibody

Formalin-fixed, paraffin-embedded human colon cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Mouse Anti-RBL2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS603691

Immunohistochemistry (IHC) of anti-RBL2 antibody

Formalin-fixed, paraffin-embedded rat spleen stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of splenic cells.
Mouse Anti-RBL2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS603691

Testing Data of anti-RBL2 antibody

IP/WS
Mouse Anti-RBL2 Monoclonal Antibody, Testing Data – Cat# MBS603691

Other Notes

Small volumes of anti-RBL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBL2 purchase

MBS603691 is an antibody targeting RBL2. Also known as Rb2, p130 (BSA & Azide Free). Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RBL2. It may not necessarily be applicable to this product.

NCBI GI #
172072597
NCBI GeneID
5934
NCBI Accession #
NP_005602.3
UniProt Primary Accession #
Q08999
UniProt Secondary Accession #
Q15073; Q16084; Q92812
UniProt Related Accession #
Q08999
GenBank Nucleotide #
NM_005611.3
NCBI Official Full Name
retinoblastoma-like protein 2
NCBI Official Synonym Full Names
retinoblastoma-like 2 (p130)
NCBI Official Symbol
RBL2
NCBI Official Synonym Symbols
Rb2; P130
UniProt Protein Name
Retinoblastoma-like protein 2
UniProt Synonym Protein Names
130 kDa retinoblastoma-associated protein; p130; Retinoblastoma-related protein 2; RBR-2; pRb2
Protein Family
Retinoblastoma-like protein
UniProt Gene Name
RBL2
UniProt Synonym Gene Names
RB2; p130; RBR-2
UniProt Entry Name
RBL2_HUMAN
NCBI Protein Information
retinoblastoma-like protein 2; PRB2; RBR-2; retinoblastoma-related protein 2; 130 kDa retinoblastoma-associated protein

UniProt Comments for RBL2

Rb-like 2: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. Belongs to the retinoblastoma protein (RB) family.

Protein type: Tumor suppressor; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of cell cycle; chromatin modification; regulation of lipid kinase activity; mitotic cell cycle
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Research Articles on RBL2

1. Low expression of RBL2 is associated with glioma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RBL2 antibody

 Reference Product  PubMed Publications
 RBL1 antibody  >172 publications with RBL2 and RBL1
 E2F4 antibody  >64 publications with RBL2 and E2F4
 CDK2 antibody  >47 publications with RBL2 and CDK2
 CCND1 antibody  >31 publications with RBL2 and CCND1
 E2F5 antibody  >14 publications with RBL2 and E2F5
 HDAC1 antibody  >11 publications with RBL2 and HDAC1

Pathways associated with anti-RBL2 antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin D Associated Events In G1 Pathway antibodies  Cyclin D Associated Events In G1 Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 FoxO Family Signaling Pathway antibodies  FoxO Family Signaling Pathway Diagram
 G0 And Early G1 Pathway antibodies  G0 And Early G1 Pathway Diagram

Diseases associated with anti-RBL2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >162 publications with RBL2 and Neoplasms
 Neoplasms, Experimental Antibodies  >22 publications with RBL2 and Neoplasms, Experimental
 Lung Diseases Antibodies  >18 publications with RBL2 and Lung Diseases
 Hyperplasia Antibodies  >10 publications with RBL2 and Hyperplasia
 Ovarian Neoplasms Antibodies  >10 publications with RBL2 and Ovarian Neoplasms
 Liver Diseases Antibodies  >7 publications with RBL2 and Liver Diseases
 Liver Neoplasms Antibodies  >7 publications with RBL2 and Liver Neoplasms
 Disease Models, Animal Antibodies  >7 publications with RBL2 and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >6 publications with RBL2 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >6 publications with RBL2 and Cardiovascular Diseases

Organs/Tissues associated with anti-RBL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >40 publications with RBL2 and Blood
 Lung Antibodies  >29 publications with RBL2 and Lung
 Brain Antibodies  >24 publications with RBL2 and Brain
 Muscle Antibodies  >22 publications with RBL2 and Muscle
 Embryonic Tissue Antibodies  >21 publications with RBL2 and Embryonic Tissue
 Heart Antibodies  >13 publications with RBL2 and Heart
 Liver Antibodies  >13 publications with RBL2 and Liver
 Eye Antibodies  >13 publications with RBL2 and Eye
 Bone Antibodies  >12 publications with RBL2 and Bone
 Nerve Antibodies  >11 publications with RBL2 and Nerve
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