Mouse Anti-NANOS2 Monoclonal Antibody – Cat# MBS603788

NANOS2 Antibody – Mouse Monoclonal

Name: NANOS2 Antibody; Monoclonal; Mouse; Hamster-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS603788
Price: 1000 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603788

Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos-type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa… More details

$1000 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Nanos2, Monoclonal Antibody

Full Product Name

Nanos2

Product Gene Name

anti-NANOS2 antibody

Product Synonym Names

Anti -Nanos2

Clonality

Monoclonal

Isotype

IgG1

Clone Number

8H151

Host

Mouse

Species Reactivity

Hamster

Form/Format

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.

Purity/Purification

Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Nanos2.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

15,132 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (46416472..46418036, complement). Location: 19q13.32

OMIM

608228

3D Structure

ModBase 3D Structure for P60321

Immunogen

E. coli-derived rhNanos2 (aa 1 - 173)

Product Categories/Family for anti-NANOS2 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

Applications Tested/Suitable for anti-NANOS2 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-NANOS2 antibody

Suitable for use in Direct ELISA and Western Blot.
Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhNanos2 is ~ 2.5ng/well.
Western Blot: 1-2ug/ml detection limit for rhNanos2 is ~ 5ng/lane under non-reducing and reducing conditions.

Other Notes

Small volumes of anti-NANOS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NANOS2 purchase

MBS603788 is an antibody targeting NANOS2. Reactive in Hamster. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NANOS2. It may not necessarily be applicable to this product.

NCBI GI #

71143121

NCBI GeneID

339345

NCBI Accession #

NP_001025032.1

UniProt Primary Accession #

P60321

UniProt Secondary Accession #

Q17R30; Q4G0P8

UniProt Related Accession #

P60321

GenBank Nucleotide #

NM_001029861.2

NCBI Official Full Name

nanos homolog 2

NCBI Official Synonym Full Names

nanos homolog 2 (Drosophila)

NCBI Official Symbol

NANOS2

NCBI Official Synonym Symbols

NOS2

UniProt Protein Name

Nanos homolog 2

Protein Family

Nanos

UniProt Gene Name

NANOS2

UniProt Synonym Gene Names

NOS2

UniProt Entry Name

NANO2_HUMAN

NCBI Protein Information

nanos homolog 2; NOS-2

UniProt Comments for NANOS2

NANOS2: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state. Belongs to the nanos family.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: mRNA binding; protein binding; zinc ion binding

Biological Process: multicellular organismal development; negative regulation of translation; negative regulation of meiosis; mRNA catabolic process; germ-line stem cell maintenance; spermatogenesis

Research Articles on NANOS2

1. Data describe for the first time the expression pattern of the NANOS2 gene in human tissues and show that it is testis specific. We found that NANOS2 protein is present in prenatal germ cells and at later stages in spermatogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NANOS2 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>12 publications with NANOS2 and Testis
Brain Antibodies	>2 publications with NANOS2 and Brain