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Neuron Cell Surface Antigen A2b5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603922

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. More details

$1045 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Neuron Cell Surface Antigen A2b5, Monoclonal Antibody
Full Product Name
Neuron Cell Surface Antigen A2b5 (A2B5 Cell Surface Ganglioside)
Product Synonym Names
Anti -Neuron Cell Surface Antigen A2b5 (A2B5 Cell Surface Ganglioside)
Clonality
Monoclonal
Isotype
IgM
Clone Number
2Q162
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Purity/Purification
Purified
Purified by ammonium sulfate precipation.
Specificity
Recognzes a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Embryonic chick retina cells.
Positive Control
Type II astrocytes, human neural progenitors, PC12 cells

Product Categories/Family for anti-Neuron Cell Surface Antigen A2b5 antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-Neuron Cell Surface Antigen A2b5 antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Neuron Cell Surface Antigen A2b5 antibody
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry.
Dilution: Indirect Immunofluorescence: 1:100-1:500. For the detection of neurons in tissue culture. Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments
Immunohistochemistry: Frozen, fixed tissues
Immunocytochemistry: Confocal fluorescent analysis of PC12 cells

Testing Data of anti-Neuron Cell Surface Antigen A2b5 antibody

Confocal fluorescent analysis of PC12 using MBS603922 (Red). Actin filaments have been labeled with AlexaFluor 488® -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining is positive.
Mouse Anti-Neuron Cell Surface Antigen A2b5 Monoclonal Antibody – Cat# MBS603922

Other Notes

Small volumes of anti-Neuron Cell Surface Antigen A2b5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Neuron Cell Surface Antigen A2b5 purchase

MBS603922 is an antibody targeting Neuron Cell Surface Antigen A2b5. Also known as Neuron Cell Surface Antigen A2b5 (A2B5 Cell Surface Ganglioside). Reactive in Human, Mouse. Host: Mouse.
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