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Heparin Binding EGF-Like Growth Factor (HBEGF) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603983

Heparin-binding EGF-like Growth Factor (HB-EGF) is an EGF family growth factor that is synthesized as a transmembrane precursor (proHB-EGF). ProHB-EGF, which contains one EGF-like domain, is subject to variable O-linked glycosylation and N-terminal… More details

$1095 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Heparin Binding EGF-like Growth Factor (HBEGF), Monoclonal Antibody
Full Product Name
Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR)
Product Gene Name
anti-HBEGF antibody
Product Synonym Names
Anti -Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H229
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Concentration
~0.5 mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes human Heparin Binding EGF-like Growth Factor (HB-EGF). Does not crossreact with recombinant human Amphiregulin, BTC, HRG-alpha, HRG-beta, EGF, TGF-alpha or recombinant mouse Epigen or Epiregulin.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Molecular Weight
23,067 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (139712428..139726188, complement). Location: 5q23
OMIM
126150
3D Structure
ModBase 3D Structure for Q99075
Immunogen
Recombinant protein corresponding to aa63-148 of human HB-EGF expressed in Sf21. Species Sequence Homology (77%): rat and mouse
Endotoxin Level
<0.1EU/ug (LAL).

Product Categories/Family for anti-HBEGF antibody

Antibodies; Abs to Growth Factors; Cytokines
Applications Tested/Suitable for anti-HBEGF antibody
ELISA (EL/EIA), Western Blot (WB), Neutralization
Application Notes for anti-HBEGF antibody
Suitable for use in Western Blot, Neutralization and ELISA.
Dilution: Western Blot: 1ug/ml using recombinant human Heparin Binding EGF-like Growth Factor.
Neutralization: ~0.5-2.5ug/ml yields one-half maximal inhibition (ND50) of the biological activity of recombinant human HB-EGF (5ng/ml). Measured by its ability to neutralize HB-EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line.

Other Notes

Small volumes of anti-HBEGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HBEGF purchase

MBS603983 is an antibody targeting HBEGF. Also known as Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HBEGF. It may not necessarily be applicable to this product.

NCBI GI #
183867
NCBI GeneID
1839
UniProt Primary Accession #
Q99075
UniProt Secondary Accession #
B2R821
UniProt Related Accession #
Q14487; Q53H93; Q99075; Q9UMJ6
NCBI Official Full Name
heparin-binding EGF-like growth factor
NCBI Official Synonym Full Names
heparin-binding EGF-like growth factor
NCBI Official Symbol
HBEGF
NCBI Official Synonym Symbols
DTR; DTS; DTSF; HEGFL
UniProt Protein Name
Proheparin-binding EGF-like growth factor
UniProt Synonym Protein Names
Diphtheria toxin receptor
Protein Family
Proheparin-binding EGF-like growth factor
UniProt Gene Name
HBEGF
UniProt Synonym Gene Names
DTR; DTS; HEGFL
UniProt Entry Name
HBEGF_HUMAN
NCBI Protein Information
proheparin-binding EGF-like growth factor; OTTHUMP00000159876; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)

UniProt Comments for HBEGF

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; wound healing, spreading of epidermal cells; positive regulation of cell migration
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Research Articles on HBEGF

1. ATP induces HB-EGF synthesis and release by interacting with the P2 purinergic receptor and through p38 and ERK1/2 signaling in response to a challenging environment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HBEGF antibody

 Reference Product  PubMed Publications
 EGFR antibody  >13 publications with HBEGF and EGFR
 ERBB4 antibody  >4 publications with HBEGF and ERBB4
 MMP7 antibody  >2 publications with HBEGF and MMP7
 CD44 antibody  >1 publications with HBEGF and CD44

Pathways associated with anti-HBEGF antibody

 Products by Pathway  Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram
 ErbB Receptor Signaling Network Pathway antibodies  ErbB Receptor Signaling Network Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB4 Signaling Events Pathway antibodies  ErbB4 Signaling Events Pathway Diagram
 GnRH Signaling Pathway antibodies  GnRH Signaling Pathway Diagram
 GnRH Signaling Pathway antibodies  GnRH Signaling Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram

Diseases associated with anti-HBEGF antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7 publications with HBEGF and Neoplasms
 Cardiovascular Diseases Antibodies  >7 publications with HBEGF and Cardiovascular Diseases
 Inflammation Antibodies  >6 publications with HBEGF and Inflammation
 Vascular Diseases Antibodies  >5 publications with HBEGF and Vascular Diseases
 Heart Diseases Antibodies  >4 publications with HBEGF and Heart Diseases
 Carcinoma Antibodies  >3 publications with HBEGF and Carcinoma
 Colorectal Neoplasms Antibodies  >3 publications with HBEGF and Colorectal Neoplasms
 Drug Toxicity Antibodies  >3 publications with HBEGF and Drug Toxicity
 Lung Diseases Antibodies  >3 publications with HBEGF and Lung Diseases
 Neoplasm Metastasis Antibodies  >2 publications with HBEGF and Neoplasm Metastasis

Organs/Tissues associated with anti-HBEGF antibody

 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >9 publications with HBEGF and Placenta
 Lung Antibodies  >8 publications with HBEGF and Lung
 Vascular Antibodies  >7 publications with HBEGF and Vascular
 Muscle Antibodies  >7 publications with HBEGF and Muscle
 Embryonic Tissue Antibodies  >6 publications with HBEGF and Embryonic Tissue
 Uterus Antibodies  >6 publications with HBEGF and Uterus
 Blood Antibodies  >5 publications with HBEGF and Blood
 Skin Antibodies  >4 publications with HBEGF and Skin
 Heart Antibodies  >3 publications with HBEGF and Heart
 Liver Antibodies  >3 publications with HBEGF and Liver
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