Blocking (CD152) Antibody – Mouse Monoclonal

Suitable for use in Flow Cytometry, ELISA, Blocking, Immunocytochemistry and Immunohistochemistry.
Dilution: Immunohistochemistry: Frozen sections
ELISA: 1ng/ml
Flow Cytometry Protocol: 1. Ficoll prepared human peripheral blood lymphocytes were stimulated in culture 3 days in the presence of PHA (5ug/ml). 2. 5x10e5 cells were washed and pre-incubated five minutes on ice with 20ul of 250ug/ml human Ig (to block nonspecific binding) and then incubated 45 minutes on ice with 80ul of C2548-83C1 at 20ug/ml.
3. Cells were washed twice and incubated with secondary reagent S7973-88P, after which they were washed three times, fixed and analyzed using a BD FACstar plus.
4. The cells stained positive with a mean shift of 2.28 log10 fluorescent units when compared to Mouse IgG1/Biotin negative control.
5. Binding was 99% blocked when cells were first pre incubated 10 minutes with 20ul of 0.5mg/ml C2548-83C antibody.