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Carboxypeptidase A2 (CPA2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604060

Carboxypeptidase A2 (CPA2) cleaves C-terminal residues with aromatic side chains More details

$1230 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carboxypeptidase A2 (CPA2), Monoclonal Antibody
Full Product Name
Carboxypeptidase A2
Product Gene Name
anti-CPA2 antibody
Product Synonym Names
Anti -Carboxypeptidase A2
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8B4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5%, canine 90%, rat 87%, mouse 86% and chicken 70%.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
47,030 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (129906703..129929638). Location: 7q32
OMIM
600688
3D Structure
ModBase 3D Structure for P48052
Immunogen
NS0-derived recombinant human rhCPA2.

Product Categories/Family for anti-CPA2 antibody

Antibodies; Abs to Enzymes; Carboxypeptidase
Applications Tested/Suitable for anti-CPA2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization
Application Notes for anti-CPA2 antibody
Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation., , Recommended Dilutions:, Western blot:1-2ug/ml the detection limit is~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold., Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50% of the enzymatic activity was inhibited at~ 1.5 ug/ml (IC50) under conditions in which the enzyme was, present at 0.1 ug/ml and the substrate concentration was 0.1 mM. Considering the, molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at, approximately 5:1 molar ratio of the antibody to the enzyme., Immunoprecipitation: 25 ug/ml, Direct ELISA: 0.5-1.0 ug/mL, Optimal dilutions to be determined bythe researcher.

Other Notes

Small volumes of anti-CPA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPA2 purchase

MBS604060 is an antibody targeting CPA2. Also known as Carboxypeptidase A2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CPA2. It may not necessarily be applicable to this product.

NCBI GI #
217416390
NCBI GeneID
1358
NCBI Accession #
NP_001860.2
UniProt Primary Accession #
P48052
UniProt Secondary Accession #
Q53XS1; Q96A12; Q96QN3; Q9UCF1; A4D1M4; C9JIK1
UniProt Related Accession #
P48052
GenBank Nucleotide #
NM_001869.2
NCBI Official Full Name
carboxypeptidase A2
NCBI Official Synonym Full Names
carboxypeptidase A2 (pancreatic)
NCBI Official Symbol
CPA2
NCBI Official Synonym Symbols
CPA2
UniProt Protein Name
Carboxypeptidase A2
Protein Family
Carbamoyl-phosphate synthase arginine-specific
UniProt Gene Name
CPA2
UniProt Entry Name
CBPA2_HUMAN
NCBI Protein Information
carboxypeptidase A2; OTTHUMP00000211315

NCBI Summary for CPA2

Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq]
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UniProt Comments for CPA2

CPA2: Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq, Dec 2008]

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.17.15

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular region

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: vacuolar protein catabolic process; proteolysis
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Research Articles on CPA2

1. Chymotrypsin C is a co-activator of human pancreatic procarboxypeptidases A1 and A2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CPA2 antibody

 Products by Pathway  Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram

Diseases associated with anti-CPA2 antibody

 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >3 publications with CPA2 and Skin Diseases
 Neoplasms Antibodies  >3 publications with CPA2 and Neoplasms
 Obesity Antibodies  >1 publications with CPA2 and Obesity
 Edema Antibodies  >1 publications with CPA2 and Edema
 Prostatic Neoplasms Antibodies  >1 publications with CPA2 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with CPA2 and Neoplasms, Experimental
 Vascular Diseases Antibodies  >1 publications with CPA2 and Vascular Diseases
 Genital Neoplasms, Female Antibodies  >1 publications with CPA2 and Genital Neoplasms, Female
 Insulin Resistance Antibodies  >1 publications with CPA2 and Insulin Resistance

Organs/Tissues associated with anti-CPA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with CPA2 and Brain
 Pancreas Antibodies  >8 publications with CPA2 and Pancreas
 Liver Antibodies  >2 publications with CPA2 and Liver
 Kidney Antibodies  >1 publications with CPA2 and Kidney
 Prostate Antibodies  >1 publications with CPA2 and Prostate
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