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DDX27 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604066

MBS604066 is an antibody targeting DDX27. Also known as DDX27 (DEAD (Asp-Glu-Ala-Asp) Box 27, dJ686N3.1, DKFZp667N057, FLJ12917, FLJ20596, FLJ22238, HSPC259, MGC1018, PP3241, RHLP, Rrp3p). Reactive in Human, Mouse. Host: Mouse. More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DDX27, Monoclonal Antibody
Full Product Name
DDX27 (DEAD (Asp-Glu-Ala-Asp) Box 27, dJ686N3.1, DKFZp667N057, FLJ12917, FLJ20596, FLJ22238, HSPC259, MGC1018, PP3241, RHLP, Rrp3p)
Product Gene Name
anti-DDX27 antibody
Product Synonym Names
Anti -DDX27 (DEAD (Asp-Glu-Ala-Asp) Box 27, dJ686N3.1, DKFZp667N057, FLJ12917, FLJ20596, FLJ22238, HSPC259, MGC1018, PP3241, RHLP, Rrp3p)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C103
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human DDX27.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
89,835 Da
3D Structure
ModBase 3D Structure for Q96GQ7
Immunogen
Partial sequence of recombinant full-length protein to human DDX27

Product Categories/Family for anti-DDX27 antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-DDX27 antibody
Western Blot (WB)
Application Notes for anti-DDX27 antibody
Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000

Testing Data of anti-DDX27 antibody

Mouse Anti-DDX27 Monoclonal Antibody – Cat# MBS604066

Western Blot (WB) of anti-DDX27 antibody

Detection of DDX27 by Western Blot. Samples: Whole cell lysate from human HeLa (H, 25ug) and mouse NIH3T3 (M, 25ug) cells. Predicted molecular weight: 89kD.
Mouse Anti-DDX27 Monoclonal Antibody, Western Blot (WB) – Cat# MBS604066

Testing Data of anti-DDX27 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DDX27 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.
Mouse Anti-DDX27 Monoclonal Antibody, Testing Data – Cat# MBS604066

Immunostaining (IS) of anti-DDX27 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX27 mAb.
Mouse Anti-DDX27 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS604066

Other Notes

Small volumes of anti-DDX27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DDX27. It may not necessarily be applicable to this product.

NCBI GI #
56203613
UniProt Primary Accession #
Q96GQ7
UniProt Secondary Accession #
Q5VXM7; Q8WYG4; Q969N7; Q96F57; Q96L97; Q9BWY9; Q9BXF0; Q9H990; Q9NWU3; A0AVB6; B7ZLY1
NCBI Official Full Name
DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX27
UniProt Synonym Protein Names
DEAD box protein 27
Protein Family
Probable ATP-dependent RNA helicase
UniProt Gene Name
DDX27
UniProt Synonym Gene Names
RHLP
UniProt Entry Name
DDX27_HUMAN

UniProt Comments for DDX27

DDX27: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: nucleus

Molecular Function: helicase activity; ATP binding

Biological Process: metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DDX27 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with DDX27 and FLNA

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