Rabbit Anti-MAP2K2 Monoclonal Antibody – Cat# MBS604178

IB: Untreated (Lanes 1 and 3) and B-Raf phosphorylated (Lanes 2 and 4) PC-12 cell lysate was probed with MBS604178 (1:5000, Lanes 1 and 2) or total anti-MEK1 (1:1000, Lanes 3 and 4). Arrow indicates MEK1 (~45kD).

MEK1, MEK2 (MAP2K2) Antibody – Rabbit Monoclonal

Name: MAP2K2 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS604178
Price: 1015 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604178

MBS604178 is an antibody targeting Map2k2. Also known as MEK1, MEK2, phosphorylated (Ser218/222, Ser222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK… More details

$1015 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MEK1, MEK2 (Map2k2), Monoclonal Antibody

Full Product Name

MEK1, MEK2, phosphorylated (Ser218/222, Ser222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mit

Product Gene Name

anti-Map2k2 antibody

Product Synonym Names

Anti -MEK1, MEK2, phosphorylated (Ser218/222, Ser222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mit

Clonality

Monoclonal

Isotype

IgG

Clone Number

4H353

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes phosphorylated MEK1/MEK2, Mr ~45kD. Species Crossreactivity: Human, mouse and rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

44,402 Da

Chromosome Location

Chromosome: 10; NC_000076.6 (81105947..81124697). Location: 10 C1; 10 39.72 cM

3D Structure

ModBase 3D Structure for Q63932

Immunogen

Synthetic peptide containing …DpSMANpSF… in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1 (KLH coupled). The immunizing sequence is highly conserved.

Product Categories/Family for anti-Map2k2 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-Map2k2 antibody

Western Blot (WB)

Application Notes for anti-Map2k2 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1:2000-1:10,000. Detects phosphorylated MEK1/2 in RIPA lysates from PC-12 cells that had been treated with 5u of B-Raf for 30 minutes at 30 degree C. Untreated and B-Raf phosphorylated PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2865-10B (1:5000) or Pab Rb anti-Mouse MEK1, NT (Map Kinase Kinase) (M2865-07) (1:1000). Proteins were visualized using a goat anti-rabbit secondary IgG labeled with HRP and a chemiluminescence detection system.

Other Notes

Small volumes of anti-Map2k2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Map2k2. It may not necessarily be applicable to this product.

NCBI GI #

545203

NCBI GeneID

26396

NCBI Accession #

AAC60678.1

UniProt Primary Accession #

Q63932

UniProt Secondary Accession #

Q9D7B0

UniProt Related Accession #

Q63932

NCBI Official Full Name

MEK2

NCBI Official Synonym Full Names

mitogen-activated protein kinase kinase 2

NCBI Official Symbol

Map2k2

NCBI Official Synonym Symbols

MK2; MEK2; Prkmk2; AA589381

UniProt Protein Name

Dual specificity mitogen-activated protein kinase kinase 2

UniProt Synonym Protein Names

ERK activator kinase 2; MAPK/ERK kinase 2

Protein Family

Dual specificity mitogen-activated protein kinase kinase

UniProt Gene Name

Map2k2

UniProt Synonym Gene Names

Mek2; Mkk2; Prkmk2; MAP kinase kinase 2; MAPKK 2; MEK 2

UniProt Entry Name

MP2K2_MOUSE

NCBI Protein Information

dual specificity mitogen-activated protein kinase kinase 2; MEK 2; MAPKK 2; MAPK/ERK kinase 2; MAP kinase kinase 2; MAP kinase/Erk kinase; ERK activator kinase 2; mitogen activated protein kinase kinase 2; protein kinase, mitogen activated, kinase 2, p45

UniProt Comments for Map2k2

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); Kinase, protein; EC 2.7.12.2; STE group; STE7 family

Cellular Component: peroxisomal membrane; Golgi apparatus; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; cell cortex; intercellular junction; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus

Molecular Function: nucleotide binding; PDZ domain binding; protein kinase activity; MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; kinase activity; ATP binding

Biological Process: regulation of axon regeneration; regulation of stress-activated MAPK cascade; activation of MAPK activity; MAPKKK cascade; positive regulation of axonogenesis; phosphorylation; protein amino acid phosphorylation

Research Articles on Map2k2

1. review focuses on the role of the MEK1 and MEK2 kinases in the development of extraembryonic tissues and in the formation of the placenta

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Map2k2 antibody

Reference Product	PubMed Publications
Map2k1 antibody	>356 publications with Map2k2 and Map2k1
Mapk3 antibody	>147 publications with Map2k2 and Mapk3
Mapk1 antibody	>146 publications with Map2k2 and Mapk1
Braf antibody	>59 publications with Map2k2 and Braf
Raf1 antibody	>14 publications with Map2k2 and Raf1
Map3k1 antibody	>8 publications with Map2k2 and Map3k1
Il17rd antibody	>1 publications with Map2k2 and Il17rd

Pathways associated with anti-Map2k2 antibody

Products by Pathway	Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram

Diseases associated with anti-Map2k2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>168 publications with Map2k2 and Neoplasms
Cardiovascular Diseases Antibodies	>37 publications with Map2k2 and Cardiovascular Diseases
Heart Diseases Antibodies	>28 publications with Map2k2 and Heart Diseases
Necrosis Antibodies	>26 publications with Map2k2 and Necrosis
Inflammation Antibodies	>24 publications with Map2k2 and Inflammation
Melanoma Antibodies	>24 publications with Map2k2 and Melanoma
Nervous System Diseases Antibodies	>21 publications with Map2k2 and Nervous System Diseases
Breast Neoplasms Antibodies	>19 publications with Map2k2 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>14 publications with Map2k2 and Neoplasm Metastasis
Neoplasm Invasiveness Antibodies	>11 publications with Map2k2 and Neoplasm Invasiveness

Organs/Tissues associated with anti-Map2k2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>78 publications with Map2k2 and Blood
Heart Antibodies	>34 publications with Map2k2 and Heart
Skin Antibodies	>32 publications with Map2k2 and Skin
Lung Antibodies	>31 publications with Map2k2 and Lung
Brain Antibodies	>25 publications with Map2k2 and Brain
Liver Antibodies	>17 publications with Map2k2 and Liver
Kidney Antibodies	>17 publications with Map2k2 and Kidney
Bone Antibodies	>16 publications with Map2k2 and Bone
Muscle Antibodies	>11 publications with Map2k2 and Muscle
Bone Marrow Antibodies	>11 publications with Map2k2 and Bone Marrow