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SEL1L Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604203

SEL1L (suppressor of lin-12-like protein) is the human ortholog of the C. elegans sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in… More details

$735 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SEL1L, Monoclonal Antibody
Full Product Name
SEL1L (Suppressor of lin-12-like Protein)
Product Gene Name
Product Synonym Names
Anti -SEL1L (Suppressor of lin-12-like Protein)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D756
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in 150mM sodium chloride, pH 7.4, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SEL1L isoforms < 88kD. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
10,746 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (81939239..82000205, complement). Location: 14q31
3D Structure
ModBase 3D Structure for Q12916
Immunogen
Recombinant human SEL1L.
Applications Tested/Suitable for anti-SEL1L antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SEL1L antibody
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.
Dilution: Immunohistochemistry: Frozen and paraffin sections.
Western Blot: 10ug/ml

Immunoprecipitation (IP) of anti-SEL1L antibody

Immunoprecipitation of total lysates from BT-474 cells followed by Western blot analysis using S0647-10L (lane 2). Lane 1:MBS604203 SEL1L; Lane 3: No lysate; Lane 4: Mw markers. |Method: Cells were lysed using a buffer containing 50mM TRIS-HCl, pH 7.4, 150mM sodium chloride, 10% glycerol, 0.5% NP40 and protease inhibitors (10ug/ml Aproteinin, 1ug/ml Leupeptin, 1ug/ml PMSF) for 10 min. at 4°C. Lysates were then centrifuged at 12,000 rpm for 10 min. at 4°C to remove insoluble material. |Protein extracts (1.5mg/sample) were immunoprecipitated for 2 hours at 4°C using 5ug of SEL1L and 20ul of protein-A/G Sepharose beads. Precipitates were then separated on 10% SDS-PAGE, transferred onto nitrocellulose membrane and probed with 10ug/ml MBS604203.
Mouse Anti-SEL1L Monoclonal Antibody – Cat# MBS604203

Testing Data of anti-SEL1L antibody

Pancreatic adenocarcinomas: (b) with and (c) without SEL1L expression.
Mouse Anti-SEL1L Monoclonal Antibody, Testing Data – Cat# MBS604203

Other Notes

Small volumes of anti-SEL1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEL1L purchase

MBS604203 is an antibody targeting SEL1L. Also known as SEL1L (Suppressor of lin-12-like Protein). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SEL1L. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
SEL1L
NCBI Official Synonym Full Names
sel-1 suppressor of lin-12-like (C. elegans)
NCBI Official Symbol
SEL1L
NCBI Official Synonym Symbols
IBD2; SEL1L1; PRO1063; SEL1-LIKE
UniProt Protein Name
Sel-1-like protein
Protein Family
UniProt Gene Name
SEL1L
UniProt Entry Name
Q12916_HUMAN
NCBI Protein Information
protein sel-1 homolog 1; sel-1L; sel-1 suppressor of lin-12-like 1; suppressor of lin-12-like protein 1; Suppressor of lin 12 (sel-1), C. elegans, homolog of

NCBI Summary for SEL1L

The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for SEL1L

SEL1L: May play a role in Notch signaling. May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Belongs to the sel-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: protein binding

Biological Process: Notch signaling pathway; ER-associated protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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