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V5 Epitope Tag Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604391

The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. More details

$650 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
V5 Epitope Tag, Monoclonal Antibody
Full Product Name
V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5)
Product Synonym Names
Anti -V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H3115
Host
Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes the V5 epitope tag. Detects recombinant proteins tagged with the epitope, some of which include transmembrane and secreted proteins. Usually, a 14aa tag is added to the recombinant proteins, although a smaller epitope of 9aa (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
Hybridoma
SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice.
Applications Tested/Suitable for anti-V5 Epitope Tag antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Radioimmunoassay (RIA)
Application Notes for anti-V5 Epitope Tag antibody
Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA: 1:1000-1:5000
Western Blot: 1:1000-1:5000
Immunohistochemistry: Frozen

Testing Data of anti-V5 Epitope Tag antibody

V5 tagged protein detected with MBS604391.
Mouse Anti-V5 Epitope Tag Monoclonal Antibody – Cat# MBS604391

Testing Data of anti-V5 Epitope Tag antibody

V5 tagged protein detected with MBS604391.
Mouse Anti-V5 Epitope Tag Monoclonal Antibody, Testing Data – Cat# MBS604391

Other Notes

Small volumes of anti-V5 Epitope Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for V5 Epitope Tag purchase

MBS604391 is an antibody targeting V5 Epitope Tag. Also known as V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5). Host: Mouse.
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