Mouse Anti-SAFB Monoclonal Antibody – Cat# MBS604806

WB: MCF-7 whole cell lysate probed with MBS604806 (0.5ug/ml).

Scaffold Attachment Factor B (SAFB) Antibody – Mouse Monoclonal

Name: SAFB Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS604806
Price: 1015 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604806

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting… More details

$1015 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Scaffold Attachment Factor B (SAFB), Monoclonal Antibody

Full Product Name

Scaffold Attachment Factor B (SAF-B, HET)

Product Gene Name

anti-SAFB antibody

Product Synonym Names

Anti -Scaffold Attachment Factor B (SAF-B, HET)

Clonality

Monoclonal

Isotype

IgG

Clone Number

4i59

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Scaffold Attachment Factor B, Mr 130kD and an additional protein, Mr 34kD. Does not crossreact with mouse.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

102,642 Da

Chromosome Location

chromosome: 19; Location: 19p13.3-p13.2

OMIM

602895

3D Structure

ModBase 3D Structure for Q15424

Immunogen

Synthetic peptide corresponding to aa345-357 (CPEARDKEDGRKF) from the human Scaffold Attachment Factor B conjugated to KLH (U72355). Sequence Homology: Mouse and human T-box protein 2, (8/11 identical aa).

Product Categories/Family for anti-SAFB antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-SAFB antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Application Notes for anti-SAFB antibody

Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Western Blot.
Dilution: Immunohistochemistry: Reported to stain SAF-B in paraf n tissue sections.
Immunocytochemistry: Reported to stain SAF-B in MCF-7 cells.
Immunoprecipitation: Reported to immunoprecipitate SAF-B from MCF-7 and HepG2 cell lysates.
Western Blot: 0.5ug/ml detects SAF-B in whole cell extracts or RIPA lysates from MCF-7 cells. MCF-7 whole cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S0430 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse* and a chemiluminescence detection system.

Other Notes

Small volumes of anti-SAFB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAFB purchase

MBS604806 is an antibody targeting SAFB. Also known as Scaffold Attachment Factor B (SAF-B, HET). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SAFB. It may not necessarily be applicable to this product.

NCBI GI #

1213639

NCBI GeneID

6294

UniProt Primary Accession #

Q15424

UniProt Secondary Accession #

O60406; Q59HH8

UniProt Related Accession #

Q15424; Q68DW3; A0AV56

NCBI Official Full Name

scaffold attachment factor B

NCBI Official Synonym Full Names

scaffold attachment factor B

NCBI Official Symbol

SAFB

NCBI Official Synonym Symbols

HAP; HET; SAFB1; SAF-B1; DKFZp779C1727; SAFB

UniProt Protein Name

Scaffold attachment factor B1

UniProt Synonym Protein Names

HSP27 estrogen response element-TATA box-binding protein

Protein Family

Scaffold attachment factor

UniProt Gene Name

SAFB

UniProt Synonym Gene Names

HAP; HET; SAFB1

UniProt Entry Name

SAFB1_HUMAN

NCBI Protein Information

scaffold attachment factor B1; SAF-B; SAB-B1; HSP27 ERE-TATA-binding protein; Hsp27 ERE-TATA binding protein; glutathione S-transferase fusion protein; HSP27 estrogen response element-TATA box-binding protein; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein

UniProt Comments for SAFB

SAFB1: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. Can inhibit cell proliferation.

Protein type: RNA-binding; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; double-stranded DNA binding; nucleotide binding; chromatin binding

Biological Process: establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; transcription, DNA-dependent; hormone metabolic process; positive regulation of transcription from RNA polymerase II promoter; growth

Research Articles on SAFB

1. Study confirms the primary role of SAFB1/SAFB2 as corepressors and also uncovers a previously unknown role for SAFB1 in the regulation of immune genes and in estrogen-mediated repression of genes.
2. The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2.
3. Importance of ERE-BP as an attenuator of normal ERalpha signaling in vivo and is a novel target for modulation by selective estrogen receptor modulators.
4. SAFB1 is not likely to be causative of the hereditary breast cancer syndrome in west Swedish breast cancer families
5. hXOR is a tumor suppressor-targeted gene and the phosphorylation of SAFB1 is regulated by OSM, which provides a molecular basis for understanding the role of SAFB1-regulated hXOR transcription in cytokine stimulation and tumorigenesis
6. Over expression of HSP27 is associated with intrahepatic cholangiocarcinoma
7. SAFB may direct the reorganization and segregation of nuclear RNA and DNA prior to endonuclease-mediated DNA cleavage.
8. REVIEW: possibility that SAFB1 and SAFB2 are novel breast tumor suppressor genes, and how they might function in this role, are discussed
9. HSP27 is a nuclear speckle component in unstressed cells in tissue culture. It is also associated with the nucleolar compartment.
10. SAFB1 interacts in pull-down assays not only with PPARgamma but also with all nuclear receptors tested

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SAFB antibody

Reference Product	PubMed Publications
SAFB antibody	>10 publications with SAFB and SAFB

Diseases associated with anti-SAFB antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>13 publications with SAFB and Neoplasms
Breast Neoplasms Antibodies	>11 publications with SAFB and Breast Neoplasms
Adenocarcinoma Antibodies	>1 publications with SAFB and Adenocarcinoma
Cell Transformation, Neoplastic Antibodies	>1 publications with SAFB and Cell Transformation, Neoplastic
Female Urogenital Diseases Antibodies	>1 publications with SAFB and Female Urogenital Diseases
Cardiovascular Diseases Antibodies	>1 publications with SAFB and Cardiovascular Diseases
Heart Diseases Antibodies	>1 publications with SAFB and Heart Diseases
Lymphoma Antibodies	>1 publications with SAFB and Lymphoma
Neoplasms, Hormone-Dependent Antibodies	>1 publications with SAFB and Neoplasms, Hormone-Dependent
Carcinoma, Hepatocellular Antibodies	>1 publications with SAFB and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SAFB antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7 publications with SAFB and Blood
Kidney Antibodies	>4 publications with SAFB and Kidney
Testis Antibodies	>3 publications with SAFB and Testis
Heart Antibodies	>2 publications with SAFB and Heart
Nerve Antibodies	>2 publications with SAFB and Nerve
Embryonic Tissue Antibodies	>2 publications with SAFB and Embryonic Tissue
Brain Antibodies	>2 publications with SAFB and Brain
Muscle Antibodies	>1 publications with SAFB and Muscle
Skin Antibodies	>1 publications with SAFB and Skin
Mammary Gland Antibodies	>1 publications with SAFB and Mammary Gland