Mouse Anti-CALCR Monoclonal Antibody – Cat# MBS604823

Validated for flow cytometry using MCF-7 cells. Dilute MBS604823 to 25ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. MCF-7 cells were stained with anti-CALCR (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.

Calcitonin Receptor (CALCR) Antibody – Mouse Monoclonal

Name: CALCR Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS604823
Price: 1055 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS604823

Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal… More details

$1055 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Calcitonin Receptor (CALCR), Monoclonal Antibody

Full Product Name

Calcitonin Receptor (CRT, CTR, CT-R, CTR1, CALCR)

Product Gene Name

anti-CALCR antibody

Product Synonym Names

Anti -Calcitonin Receptor (CRT, CTR, CT-R, CTR1, CALCR)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

9j1

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a sterile-filtered lyophilized powder in PBS, pH 7.2, 5% trehalose. Reconstitution: sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 0.5mg/ml.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human calcitonin receptor. Stains human CALCR transfectants but not irrelevant transfectants.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

57,303 Da

Chromosome Location

Chromosome: 7; NC_000007.13 (93053799..93204042, complement). Location: 7q21.3

OMIM

114131

3D Structure

ModBase 3D Structure for P30988

Immunogen

Human CALCR (Accession # P79222)

Hybridoma

Mouse myeloma with B cells obtained from a mouse immunized with HEK293 cells transfected with human CALCR (Accession # P79222).

Product Categories/Family for anti-CALCR antibody

Antibodies; Abs to Receptors

Applications Tested/Suitable for anti-CALCR antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-CALCR antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 0.25ug/ml. Validated for flow cytometry using MCF-7 cells. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome.
MCF-7 cells were stained with anti-CALCR (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.

Other Notes

Small volumes of anti-CALCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CALCR purchase

MBS604823 is an antibody targeting CALCR. Also known as Calcitonin Receptor (CRT, CTR, CT-R, CTR1, CALCR). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CALCR. It may not necessarily be applicable to this product.

NCBI GI #

1117795

NCBI GeneID

799

UniProt Primary Accession #

P30988

UniProt Secondary Accession #

O14585; Q13941; Q6DJU8; Q6T712

UniProt Related Accession #

P30988; Q5ZGL8; Q659U6

NCBI Official Full Name

calcitonin receptor

NCBI Official Synonym Full Names

calcitonin receptor

NCBI Official Symbol

CALCR

NCBI Official Synonym Symbols

CRT; CTR; CT-R; CTR1

UniProt Protein Name

Calcitonin receptor

Protein Family

Calcitonin receptor

UniProt Gene Name

CALCR

UniProt Entry Name

CALCR_HUMAN

NCBI Protein Information

calcitonin receptor; OTTHUMP00000162535; OTTHUMP00000208683; OTTHUMP00000208686

NCBI Summary for CALCR

This gene encodes a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

UniProt Comments for CALCR

CALCR: This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin. Belongs to the G-protein coupled receptor 2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; calcitonin binding; receptor activity; protein transporter activity; calcitonin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; protein transport; phospholipase C activation; receptor internalization; positive regulation of cAMP biosynthetic process; response to glucocorticoid stimulus; adenylate cyclase activation; positive regulation of adenylate cyclase activity; G-protein signaling, adenylate cyclase activating pathway

Disease: Osteoporosis

Research Articles on CALCR

1. Allele C in CALCR gene was associated with reduced bone mass in cystic fibrosis children.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CALCR antibody

Reference Product	PubMed Publications
CALCA antibody	>2 publications with CALCR and CALCA

Pathways associated with anti-CALCR antibody

Products by Pathway	Pathway Diagram
Calcitonin-like Ligand Receptors Pathway antibodies	Calcitonin-like Ligand Receptors Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class B Secretin-like Pathway antibodies	GPCRs, Class B Secretin-like Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram

Diseases associated with anti-CALCR antibody

Disease Name	Pubmed Publications
Osteoporosis Antibodies	>6 publications with CALCR and Osteoporosis
Lung Diseases Antibodies	>4 publications with CALCR and Lung Diseases
Fibrosis Antibodies	>3 publications with CALCR and Fibrosis
Inflammation Antibodies	>2 publications with CALCR and Inflammation
Neoplasms Antibodies	>1 publications with CALCR and Neoplasms
Pancreatic Neoplasms Antibodies	>1 publications with CALCR and Pancreatic Neoplasms
Carcinoma Antibodies	>1 publications with CALCR and Carcinoma
Anemia Antibodies	>1 publications with CALCR and Anemia
Osteoporosis, Postmenopausal Antibodies	>1 publications with CALCR and Osteoporosis, Postmenopausal
Autoimmune Diseases Antibodies	>1 publications with CALCR and Autoimmune Diseases

Organs/Tissues associated with anti-CALCR antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>16 publications with CALCR and Bone
Brain Antibodies	>2 publications with CALCR and Brain
Kidney Antibodies	>1 publications with CALCR and Kidney