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5'-Nucleotidase (NT5E) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605214

5'-nucleotidase (CD73) is a 70kD GPI-anchored cell surface molecule and belongs to the 5'-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain… More details

$1230 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
5'-nucleotidase (NT5E), Monoclonal Antibody
Full Product Name
5'-nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT, E5NT, eN, eNT, NT, NT5, NTE)
Product Gene Name
anti-NT5E antibody
Product Synonym Names
Anti -5'-nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT, E5NT, eN, eNT, NT, NT5, NTE)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9G246
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes membrane bound and soluble human 5'-nucleotidase. Causes a reduction in 5'-nucleotidase expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.
Preparation and Storage
-20 degree C
Molecular Weight
63,368 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (86159302..86205498). Location: 6q14-q21
OMIM
129190
3D Structure
ModBase 3D Structure for P21589
Immunogen
Inflamed synovial stroma from rheumatoid arthitis patients.

Product Categories/Family for anti-NT5E antibody

Antibodies; Abs to Enzymes; Nucleotidase
Applications Tested/Suitable for anti-NT5E antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-NT5E antibody
Dilution: Immunofluorescence: HUVEC cells were seeded on gelatin-coated coverslips and stained with N7100-71C (5).
Flow Cytometry: 1:50. Stains the extracellular domain (1).
Immunohistochemistry (Frozen): 1:50. Tissue sections were fixed in acetone. Not suitable for use with paraffin sections (5).
Functional Studies: Functions as an inhibitor of lymphocyte binding to HUVEC. Tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer (1).
Postitive Control: HUVEC and fresh lymphocytes
Negative Control: COS cells
Storage and Stability:
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS) of anti-NT5E antibody

FC: Flow cytometric detection of human CD73 on Huvec using MBS605214. Black, red and blue line represents cells only, isotype control and 4G4 (4ug/ml, respectively).
Mouse Anti-NT5E Monoclonal Antibody – Cat# MBS605214

Other Notes

Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NT5E purchase

MBS605214 is an antibody targeting NT5E. Also known as 5'-nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT, E5NT, eN, eNT, NT, NT5, NTE). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.

NCBI GI #
23897
NCBI GeneID
4907
UniProt Primary Accession #
P21589
UniProt Secondary Accession #
O75520; Q5W116
UniProt Related Accession #
P21589; Q53Z63; Q5JRQ2; Q6NZX3; Q96B60
NCBI Official Full Name
5'-nucleotidase
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase
Protein Family
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE
UniProt Entry Name
5NTD_HUMAN
NCBI Protein Information
5'-nucleotidase; 5'-NT; OTTHUMP00000016808; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase

NCBI Summary for NT5E

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
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UniProt Comments for NT5E

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Nucleotide Metabolism - purine; Membrane protein, GPI anchor; Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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Research Articles on NT5E

1. CD73 is expressed on a subpopulation of afferent lymph vessels but is absent on efferent lymphatics, unlike LYVE-1 and podoplanin, which are expressed on both types of lymphatics.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NT5E antibody

 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Catabolism Pathway antibodies  Pyrimidine Catabolism Pathway Diagram

Diseases associated with anti-NT5E antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with NT5E and Neoplasms
 Inflammation Antibodies  >3 publications with NT5E and Inflammation
 Breast Neoplasms Antibodies  >3 publications with NT5E and Breast Neoplasms
 Pain Antibodies  >3 publications with NT5E and Pain
 Neoplasm Metastasis Antibodies  >2 publications with NT5E and Neoplasm Metastasis
 Carcinoma Antibodies  >2 publications with NT5E and Carcinoma
 Carcinoma, Squamous Cell Antibodies  >1 publications with NT5E and Carcinoma, Squamous Cell
 Leukemia Antibodies  >1 publications with NT5E and Leukemia
 Head and Neck Neoplasms Antibodies  >1 publications with NT5E and Head and Neck Neoplasms
 Hyperalgesia Antibodies  >1 publications with NT5E and Hyperalgesia

Organs/Tissues associated with anti-NT5E antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >6 publications with NT5E and Vascular
 Blood Antibodies  >4 publications with NT5E and Blood
 Muscle Antibodies  >3 publications with NT5E and Muscle
 Intestine Antibodies  >2 publications with NT5E and Intestine
 Skin Antibodies  >2 publications with NT5E and Skin
 Prostate Antibodies  >1 publications with NT5E and Prostate
 Ganglia Antibodies  >1 publications with NT5E and Ganglia
 Mouth Antibodies  >1 publications with NT5E and Mouth
 Embryonic Tissue Antibodies  >1 publications with NT5E and Embryonic Tissue
 Uterus Antibodies  >1 publications with NT5E and Uterus
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