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T-Complex Protein 1 Theta (CCT8) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605258

MBS605258 is an antibody targeting CCT8. Also known as T-complex Protein 1 theta (T-complex Protein 1 theta Subunit, TCP1 theta, TCP1-theta, TCP-1 theta, Chaperonin Containing TCP1 theta, Chaperonin-containing TCP-1 theta, CCT theta, CCT-theta, CCTQ,… More details

$705 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
T-complex Protein 1 theta (CCT8), Monoclonal Antibody
Full Product Name
T-complex Protein 1 theta (T-complex Protein 1 theta Subunit, TCP1 theta, TCP1-theta, TCP-1 theta, Chaperonin Containing TCP1 theta, Chaperonin-containing TCP-1 theta, CCT theta, CCT-theta, CCTQ, Chaperonin Containing TCP1 Subunit 8, Chaperonin-containing
Product Gene Name
Product Synonym Names
Anti -T-complex Protein 1 theta (T-complex Protein 1 theta Subunit, TCP1 theta, TCP1-theta, TCP-1 theta, Chaperonin Containing TCP1 theta, Chaperonin-containing TCP-1 theta, CCT theta, CCT-theta, CCTQ, Chaperonin Containing TCP1 Subunit 8, Chaperonin-containing
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10B492
Host
Rat
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes the theta polypeptide of the human CCT chaperonin molecule complex at ~60kD. Species Crossreactivity: mouse, rabbit, bovine
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
59,621 Da
Chromosome Location
Chromosome: 21; NC_000021.8 (30428647..30446010, complement). Location: 21q22.11
3D Structure
ModBase 3D Structure for P50990
Immunogen
CCT Theta.
Applications Tested/Suitable for anti-CCT8 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CCT8 antibody
Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Western Blot: 1:250-1:500

Western Blot (WB) of anti-CCT8 antibody

Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with RAT ANTI CCT THETA followed by F(ab')2 RABBIT ANTI RAT IgG:HRP
Rat Anti-CCT8 Monoclonal Antibody – Cat# MBS605258

Other Notes

Small volumes of anti-CCT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCT8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-complex protein 1 subunit theta
NCBI Official Synonym Full Names
chaperonin containing TCP1, subunit 8 (theta)
NCBI Official Symbol
CCT8
NCBI Official Synonym Symbols
Cctq; PRED71; D21S246; KIAA0002; C21orf112
UniProt Protein Name
T-complex protein 1 subunit theta
UniProt Synonym Protein Names
CCT-theta; Renal carcinoma antigen NY-REN-15
UniProt Gene Name
CCT8
UniProt Synonym Gene Names
C21orf112; CCTQ; KIAA0002
UniProt Entry Name
TCPQ_HUMAN
NCBI Protein Information
T-complex protein 1 subunit theta; CCT-theta; TCP-1-theta; OTTHUMP00000096462; OTTHUMP00000096463; OTTHUMP00000096464; renal carcinoma antigen NY-REN-15; T-complex protein 1, theta subunit

NCBI Summary for CCT8

This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1. [provided by RefSeq, Sep 2013]
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UniProt Comments for CCT8

CCT-theta: a chaperone of the TCP-1 chaperonin family. A part of a hetero-oligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

Protein type: Chaperone; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: chaperonin-containing T-complex; centrosome; microtubule; intermediate filament cytoskeleton; cytoplasm; cytosol

Molecular Function: protein binding; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; pore complex biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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