V5 Epitope Tag Antibody – Mouse Monoclonal

Monoclonal

IgG2a

3H3115

Mouse

Purified IgG conjugated to Biotin. Supplied as a liquid with 0.1% sodium azide.

Affinity Purified
Purified by Protein G affinity chromatography.

Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.

-20 degree C

Mice were infected with the paramyxovirus SV5, Simian-Virus 5.

Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.

Antibodies; Abs to Infectious Disease