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Cytokeratin 5,6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605301

The cytokeratins are a family of intermediate filaments fond in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or… More details

$1055 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 5,6, Monoclonal Antibody
Full Product Name
Cytokeratin 5,6
Product Synonym Names
Anti -Cytokeratin 5,6
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q1071
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity/Purification
Purified
Purified
Specificity
Recognizes human cytokeratin 5. Demonstrates crossreactivity with cytokeratin 6. Demonstrates weak crossreactivity with cytokeratin 4 on immunoblots. Does not crossreact with cytokeratins 1, 7, 8, 10, 13, 14, 18 or 19. Recognizes basal cells and a part of stratum spinosum in the normal pavement epithelium.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified cytokeratin 5
Applications Tested/Suitable for anti-Cytokeratin 5,6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Cytokeratin 5,6 antibody
Suitable for use in Immunohistochemistry, Western Blot, Immunofluorescence.
Dilution: Immunohistochemistry (frozen or paraffin sections): 1:50-1:100
Western Blot: Recognizes a 58kD and 56kD band corresponding to cytokeratin 5 and 6. Cytoskeletal preparations of epidermis.
Optimal dilution determined by the researcher.

Testing Data of anti-Cytokeratin 5,6 antibody

1:500 dilution of this lot detected MBS605301 on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS605301 (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein MBS605301 (~60/70kD). Expected MW is 62, observed 56-70 MW for cytokeratin 5 & 6
Mouse Anti-Cytokeratin 5,6 Monoclonal Antibody – Cat# MBS605301

Other Notes

Small volumes of anti-Cytokeratin 5,6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cytokeratin 5,6 purchase

MBS605301 is an antibody targeting Cytokeratin 5,6. Reactive in Human. Host: Mouse.
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