• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

F4/80, Pan Macrophages Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605325

Mouse F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other… More details

$980 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F4/80, Pan Macrophages, Monoclonal Antibody
Full Product Name
F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC)
Product Synonym Names
Anti -F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
BM8
Host
Rat
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse F4/80, a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. F0001-51B is a macrophage marker capable of distinguishing non-destructive from destructive inflammation processes in the pancreas. It is also a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Species Crossreactivity: human (heart macrophages). Does not crossreact with mouse granulocytes, mast cells, platelets, lymphocytes, fibroblasts, or endothelial cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Immunogen
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
Positive Control
Mouse macrophages

Product Categories/Family for anti-F4/80, Pan Macrophages antibody

Antibodies; Abs to Carbohydrates; Glycoproteins
Applications Tested/Suitable for anti-F4/80, Pan Macrophages antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-F4/80, Pan Macrophages antibody
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Dilution: Flow Cytometry: 1:50, Fix with 1% paraformaldehyde (3).
Western Blot: 1:50, Mouse bone-marrow derived macrophages; non-reduced. Detects a band at ~125kD (3). Reduction with 2-mercaptoethanol destroys the antigen.
Immunohistochemistry (frozen): 1:50, Tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT. Incubation with 0.02M sodium azide in PBS, 0.1% H2O2 for 10min at RT to destroy endogenous peroxidase. Use spleen as positive control
Immunohistochemistry (paraffin): 1:50, Fixation in 10% neutral buffered formalin for 24 hours; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer. Use splenic macrophages as positive control (5).

Testing Data of anti-F4/80, Pan Macrophages antibody

F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with MBS605325.
Rat Anti-F4/80, Pan Macrophages Monoclonal Antibody – Cat# MBS605325

Other Notes

Small volumes of anti-F4/80, Pan Macrophages antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F4/80, Pan Macrophages purchase

MBS605325 is an antibody targeting F4/80, Pan Macrophages. Also known as F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC). Reactive in Human, Mouse. Host: Rat.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000