• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Indoleamine 2,3-Dioxygenase (IDO1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605331

Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which… More details

$995 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Indoleamine 2,3-Dioxygenase (Ido1), Monoclonal Antibody
Full Product Name
Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)
Product Gene Name
anti-Ido1 antibody
Product Synonym Names
Anti -Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)
Clonality
Monoclonal
Isotype
IgG
Clone Number
4C2
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G chromatography.
Specificity
Recognizes human Indoleamine 2,3-Dioxygenase (IDO), Mr 42kD. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Molecular Weight
45,641 Da
Chromosome Location
Chromosome: 8; NC_000074.5 (25694613..25707481, complement). Location: 8 A2; 8
3D Structure
ModBase 3D Structure for P28776
Immunogen
GST fusion protein corresponding to aa78-184 of human Indoleamine 2,3-Dioxygenase (IDO).
Positive Control
Human IFN-gamma stimulated HeLa cell lysates,
IFN-gamma stimulated human peripheral blood lymphocytes

Product Categories/Family for anti-Ido1 antibody

Antibodies; Abs to Enzymes; Oxidase
Applications Tested/Suitable for anti-Ido1 antibody
Western Blot (WB)
Application Notes for anti-Ido1 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500 detects IDO on 10ug of untreated and IFN gamma treated Hela lysates. Untreated and IFN-gamma 24-hour-treated Hela lysates were resolved by electrophoresis, transferred to membrane and probed with I7545-01. Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.

Western Blot (WB) of anti-Ido1 antibody

WB: Unstimulated (lane 1) and human IFN-gamma stimulated (lane 2) HeLa cell lysates were probed with MBS605331 (0.5ug/ml). Arrow indicates IDO.
Mouse Anti-IDO1 Monoclonal Antibody – Cat# MBS605331

Other Notes

Small volumes of anti-Ido1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ido1 purchase

MBS605331 is an antibody targeting Ido1. Also known as Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Ido1. It may not necessarily be applicable to this product.

NCBI GI #
194047
NCBI GeneID
15930
UniProt Primary Accession #
P28776
UniProt Related Accession #
P28776
NCBI Official Full Name
indoleamine 2,3-dioxygenase
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
Ido1
NCBI Official Synonym Symbols
Ido; Indo
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Synonym Protein Names
Indoleamine-pyrrole 2,3-dioxygenase
Protein Family
Indoleamine 2,3-dioxygenase
UniProt Gene Name
Ido1
UniProt Synonym Gene Names
Ido; Indo
UniProt Entry Name
I23O1_MOUSE
NCBI Protein Information
indoleamine 2,3-dioxygenase 1; IDO-1; OTTMUSP00000022551; OTTMUSP00000022552; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase

UniProt Comments for Ido1

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Cell cycle regulation; Amino Acid Metabolism - tryptophan; EC 1.13.11.52; Oxidoreductase

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytoplasm; cytosol

Molecular Function: amino acid binding; protein binding; dioxygenase activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; oxidoreductase activity; oxygen binding; tryptophan 2,3-dioxygenase activity

Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; positive regulation of apoptosis; negative regulation of activated T cell proliferation; response to lipopolysaccharide; defense response; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; inflammatory response; tryptophan catabolic process; multicellular organismal response to stress; positive regulation of T cell tolerance induction
Show more

Research Articles on Ido1

1. The authors demonstrate an increase in the rate of colonic epithelial cell turnover after inhibition of IDO in Trichuris muris-infected SCID mice, leading to a significant expulsion of parasite burden.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Ido1 antibody

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Tryptophan Catabolism Pathway antibodies  Tryptophan Catabolism Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram

Diseases associated with anti-Ido1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with Ido1 and Inflammation
 Neoplasms Antibodies  >5 publications with Ido1 and Neoplasms
 Sepsis Antibodies  >2 publications with Ido1 and Sepsis
 Autoimmune Diseases Antibodies  >2 publications with Ido1 and Autoimmune Diseases
 Carcinoma Antibodies  >2 publications with Ido1 and Carcinoma
 Melanoma Antibodies  >1 publications with Ido1 and Melanoma
 Neoplasm Metastasis Antibodies  >1 publications with Ido1 and Neoplasm Metastasis
 Drug Toxicity Antibodies  >1 publications with Ido1 and Drug Toxicity
 Neoplasm Invasiveness Antibodies  >1 publications with Ido1 and Neoplasm Invasiveness
 Pancreatic Neoplasms Antibodies  >1 publications with Ido1 and Pancreatic Neoplasms

Organs/Tissues associated with anti-Ido1 antibody

 Organ/Tissue Name  Pubmed Publications
 Epididymis Antibodies  >1 publications with Ido1 and Epididymis
 Intestine Antibodies  >1 publications with Ido1 and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000