Mouse Anti-POLR3E Monoclonal Antibody – Cat# MBS605348

POLR3E Antibody – Mouse Monoclonal

Name: POLR3E Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS605348
Price: 850 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605348

MBS605348 is an antibody targeting POLR3E. Also known as POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E, DNA Directed RNA Polymerase III 80kD Polypeptide, FLJ10509, KIAA1452, RNA Polymerase III 80kD Subunit RPC5, RNA Polymerase III Subunit C5).… More details

$850 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

POLR3E, Monoclonal Antibody

Full Product Name

POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E, DNA Directed RNA Polymerase III 80kD Polypeptide, FLJ10509, KIAA1452, RNA Polymerase III 80kD Subunit RPC5, RNA Polymerase III Subunit C5)

Product Gene Name

anti-POLR3E antibody

Product Synonym Names

Anti -POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E, DNA Directed RNA Polymerase III 80kD Polypeptide, FLJ10509, KIAA1452, RNA Polymerase III 80kD Subunit RPC5, RNA Polymerase III Subunit C5)

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

9j73

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.05% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human POLR3E.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

79,967 Da

Chromosome Location

Chromosome: 16; NC_000016.9 (22308741..22345346). Location: 16p12.2

3D Structure

ModBase 3D Structure for Q53GZ2

Immunogen

Synthetic peptide corresponding to the middle region of human POLR3E

Product Categories/Family for anti-POLR3E antibody

Antibodies; Abs to Enzymes; Polymerase

Applications Tested/Suitable for anti-POLR3E antibody

Western Blot (WB)

Application Notes for anti-POLR3E antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-POLR3E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR3E. It may not necessarily be applicable to this product.

NCBI GI #

12653043

NCBI GeneID

55718

UniProt Primary Accession #

Q53GZ2

UniProt Related Accession #

Q53GZ2; Q9NVU0

NCBI Official Full Name

POLR3E protein

NCBI Official Synonym Full Names

polymerase (RNA) III (DNA directed) polypeptide E (80kD)

NCBI Official Symbol

POLR3E

NCBI Official Synonym Symbols

SIN; RPC5

UniProt Protein Name

Polymerase (RNA) III (DNA directed) polypeptide E variant

Protein Family

DNA-directed RNA polymerase

UniProt Entry Name

Q53GZ2_HUMAN

NCBI Protein Information

DNA-directed RNA polymerase III subunit RPC5; OTTHUMP00000120008; RNA polymerase III subunit C5; RNA polymerase III 80 kDa subunit RPC5; DNA-directed RNA polymerase III 80 kDa polypeptide

UniProt Comments for POLR3E

POLR3E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex; Transferase; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: nucleoplasm; centrosome; DNA-directed RNA polymerase III complex; nucleus; cytosol

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

Research Articles on POLR3E

1. Data describe the purification and identification of RNA polymerase III subunits RPC2 and RPC5.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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