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Galectin-14 (LGALS14) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605811

Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14… More details

$995 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Galectin-14 (LGALS14), Monoclonal Antibody
Full Product Name
Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13)
Product Gene Name
Product Synonym Names
Anti -Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9H126
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
Purity/Purification
Affinity Purified
Purified by Protein G immunoaffinity chromatography.
Specificity
Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
16,094 Da
Chromosome Location
chromosome: 19; Location: 19q13.2
OMIM
607260
3D Structure
ModBase 3D Structure for Q8TCE9
Immunogen
Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14; aa 1-139; Accession# Q8TCE9).

Product Categories/Family for anti-LGALS14 antibody

Applications Tested/Suitable for anti-LGALS14 antibody
Western Blot (WB)
Application Notes for anti-LGALS14 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20.
Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well.

Other Notes

Small volumes of anti-LGALS14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGALS14 purchase

MBS605811 is an antibody targeting LGALS14. Also known as Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LGALS14. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
galectin-14 transcription variant 1
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 14
NCBI Official Symbol
LGALS14
NCBI Official Synonym Symbols
CLC2; PPL13; MGC22235; LGALS14
UniProt Protein Name
Placental protein 13-like
UniProt Synonym Protein Names
Charcot-Leyden crystal protein 2; CLC2; Galectin-14
Protein Family
UniProt Gene Name
LGALS14
UniProt Synonym Gene Names
PPL13
UniProt Entry Name
PPL13_HUMAN
NCBI Protein Information
placental protein 13-like; gal-14; galectin-14; Charcot-Leyden crystal protein 2; placental protein 13-like protein

NCBI Summary for LGALS14

This gene is predominantly expressed in placenta. The encoded protein belongs to the galectin (galaptin/S-lectin) family. The members of galectin family contain one or two carbohydrate recognition domains, which can bind beta-galactoside. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
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UniProt Comments for LGALS14

LGALS14: 2 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleus

Molecular Function: carbohydrate binding

Biological Process: apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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