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Tubulin, Gamma Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS606699

Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC,… More details

$705 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Tubulin, gamma, Monoclonal Antibody
Full Product Name
Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam) (Dyomics 547)
Product Synonym Names
Anti -Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam) (Dyomics 547)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9F154
Host
Mouse
Species Reactivity
Chicken, Human, Mouse, Porcine, Rat, Xenopus
Form/Format
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Dyomics 547.
Purity/Purification
Purified
Purified
Specificity
Recognizes human gamma tubulin in stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Dyomics 547 conjugates are sensitive to light.
Immunogen
Synthetic peptide corresponding to aa434-449, EYHAATRPDYISWGTQ representing the C-Terminus of the human protein of gamma Tubulin (KLH).

Product Categories/Family for anti-Tubulin, gamma antibody

Antibodies; Abs to Tubulin
Applications Tested/Suitable for anti-Tubulin, gamma antibody
Immunocytochemistry (ICC)
Application Notes for anti-Tubulin, gamma antibody
Suitable for use in Immunocytochemistry.
Dilution: Immunocytochemistry: 1:500. Samples need to be fixed and permeabilized

Immunofluorescence (IF) of anti-Tubulin, gamma antibody

Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with MBS606699, alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).
Mouse Anti-Tubulin, Gamma Monoclonal Antibody – Cat# MBS606699

Immunofluorescence (IF) of anti-Tubulin, gamma antibody

Immunofluorescence staining of mouse fibroblasts MBS606699 (direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).
Mouse Anti-Tubulin, Gamma Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS606699

Immunofluorescence (IF) of anti-Tubulin, gamma antibody

Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using MBS606699 (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).
Mouse Anti-Tubulin, Gamma Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS606699

Other Notes

Small volumes of anti-Tubulin, gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tubulin, gamma purchase

MBS606699 is an antibody targeting Tubulin, gamma. Also known as Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam) (Dyomics 547). Reactive in Chicken, Human, Mouse, Porcine, Rat, Xenopus. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Tubulin, gamma. It may not necessarily be applicable to this product.

NCBI GI #
4115724
NCBI Accession #
BAA36504.1
NCBI Official Full Name
tubulin
Protein Family
Tubulin

UniProt Comments for Tubulin, gamma

TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: centrosome; pericentriolar material; condensed nuclear chromosome; nonmotile primary cilium; leading edge; polar microtubule; cilium; gamma-tubulin complex; centriole; recycling endosome; cytoplasmic microtubule; apical part of cell; spindle microtubule; cytoplasm; microtubule organizing center

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; microtubule nucleation; meiotic spindle organization and biogenesis; cytoplasmic microtubule organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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