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Tropoelastin, Ligamentum Nuchae Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS606788

Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an… More details

$650 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tropoelastin, Ligamentum Nuchae, Monoclonal Antibody
Full Product Name
Tropoelastin, Ligamentum Nuchae
Product Synonym Names
Anti -Tropoelastin, Ligamentum Nuchae
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G3
Host
Mouse
Species Reactivity
Bovine
Form/Format
Supplied as a lyophilized powder. No preservatives added. Reconstitute with 200ul sterile ddH2O. Dilution with 50% glycerol is preferred.
Purity/Purification
Ascites
Specificity
Recognizes bovine tropoelastin and insoluble forms of elastin. Low Species Crossreactivity: human, rabbit, porcine and sheep. This antibody show little if any reactivity towards rat or chicken elastins.
Preparation and Storage
Lyophilized and receonstituted products are stable for 12 months after receipt at -20°C. Reconstitute with stable ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Bovine ligamentum nuchae tropoelastin.
Grade
Ascites
Applications Tested/Suitable for anti-Tropoelastin, Ligamentum Nuchae antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000 -1:2000
Western Blot: 1:200 -1:500
Immunoprecipitation: 1:100-1:200
Immunohistochemistry: 1:50 -1:100
Immunocytochemistry: 1:50-1:100

Optimal dilutions to be determined by the researcher.


Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine-HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.

Other Notes

Small volumes of anti-Tropoelastin, Ligamentum Nuchae antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tropoelastin, Ligamentum Nuchae purchase

MBS606788 is an antibody targeting Tropoelastin, Ligamentum Nuchae. Reactive in Bovine. Host: Mouse.
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