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Fibroblast Growth Factor-21 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS606888

Fibroblast Growth Factor-21 (FGF-21) belongs to the FGF family of heparin-binding polypeptides and is most closely related to FGF-23. Both proteins are synthesized as a precursor with hydrophobic signal peptides. FGF-21 is most highly expressed in the liver.… More details

$1045 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Growth Factor-21, Monoclonal Antibody
Full Product Name
Fibroblast Growth Factor-21 (FGF-21) (UNQ3115/PRO10196)
Product Synonym Names
Anti -Fibroblast Growth Factor-21 (FGF-21) (UNQ3115/PRO10196)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9H123
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Fibroblast Growth Factor-21 (FGF-21). Does not crossreact with recombinant human FGF-5 or recombinant human FGF-9. Shows <10% crossreactivity with recombinant human FGF-3, 4, 6 and 10. Species Crossreactivity: mouse <10%.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Immunogen
Recombinant corresponding to aa29-209 of human FGF-21 expressed in E. coli (Q9NSA1).
Applications Tested/Suitable for anti-Fibroblast Growth Factor-21 antibody
ELISA (EL/EIA), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Suitable for use in Flow Cytometry, Immunocytochemistry and ELISA., , Recommended Dilution:, Flow Cytometry: 25ug/ml; 10ul labels 1-5x10e5 HepG2 cells in a total reaction volume <200ul. For intercellular staining, cells must be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin., Immunocytochemistry: 10ug/ml; HepG2 human cells were fixed PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-10, 1% BSA for 45 min at RT. Cells were incubated with F4212-93E overnight at 4ºC follwed by IgG (FITC) anti-mouse* at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step., Direct ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well., Optimal dilutions to be determined by the researcher.

Testing Data of anti-Fibroblast Growth Factor-21 antibody

HepG2 cells were stained with MBS606888 (filled histogram) or isotype control (open histogram) followed by |APC-conjugated anti-mouse antibody.
Mouse Anti-Fibroblast Growth Factor-21 Monoclonal Antibody – Cat# MBS606888

Other Notes

Small volumes of anti-Fibroblast Growth Factor-21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor-21 purchase

MBS606888 is an antibody targeting Fibroblast Growth Factor-21. Also known as Fibroblast Growth Factor-21 (FGF-21) (UNQ3115/PRO10196). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Fibroblast Growth Factor-21. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
fibroblast growth factor 21

NCBI Summary for Fibroblast Growth Factor-21

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for Fibroblast Growth Factor-21

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Fibroblast Growth Factor-21 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Fibroblast Growth Factor-21 and FLNA
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