Rat Anti-HAVCR2 Monoclonal Antibody – Cat# MBS606914

Whole blood monocytes were stained with MBS606914 (filled histogram) or isotype control (open histogram).

HAVCR2 Antibody – Rat Monoclonal

Name: HAVCR2 Antibody; Monoclonal; Rat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS606914
Price: 1110 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS606914

T cell immunoglobulin mucin-3 (Tim3) is a member of the T cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. Tim3 is selectively expressed on Th1 cells and interacts with… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tim3 (HAVCR2), Monoclonal Antibody

Full Product Name

Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)

Product Gene Name

anti-HAVCR2 antibody

Product Synonym Names

Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)

Clonality

Monoclonal

Isotype

IgG2A

Clone Number

9L856

Host

Rat

Species Reactivity

Human

Form/Format

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC)

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Concentration

0.01 mg/ml (lot specific)

Specificity

Recognizes human Tim3.

Preparation and Storage

May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.

Molecular Weight

33,394 Da

Chromosome Location

chromosome: 5; Location: 5q33.3

OMIM

606652

3D Structure

ModBase 3D Structure for Q8TDQ0

Immunogen

Recombinant protein corresponding to aa22-200 from the extracellular domain of human Tim3 expressed in NSO cells (NP_116171).

Product Categories/Family for anti-HAVCR2 antibody

Antibodies; Abs to T Cells

Applications Tested/Suitable for anti-HAVCR2 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-HAVCR2 antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated using whole blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of T5469-92F to up to 1x10e6 cells and incubate for 30 minutes at RT
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2A antibody.

Other Notes

Small volumes of anti-HAVCR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAVCR2 purchase

MBS606914 is an antibody targeting HAVCR2. Also known as Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC). Reactive in Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for HAVCR2. It may not necessarily be applicable to this product.

NCBI GI #

18182535

NCBI GeneID

84868

UniProt Primary Accession #

Q8TDQ0

UniProt Secondary Accession #

Q8WW60; Q96K94; B2RAY2

UniProt Related Accession #

Q8TDQ0

NCBI Official Full Name

T cell immunoglobulin mucin-3

NCBI Official Synonym Full Names

hepatitis A virus cellular receptor 2

NCBI Official Symbol

HAVCR2

NCBI Official Synonym Symbols

TIM3; KIM-3; TIMD3; Tim-3; FLJ14428; HAVCR2

UniProt Protein Name

Hepatitis A virus cellular receptor 2

UniProt Synonym Protein Names

T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell membrane protein 3; TIM-3

Protein Family

Hepatitis A virus cellular receptor

UniProt Gene Name

HAVCR2

UniProt Synonym Gene Names

TIM3; TIMD3

UniProt Entry Name

HAVR2_HUMAN

NCBI Protein Information

hepatitis A virus cellular receptor 2; TIMD-3; HAVcr-2; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3

NCBI Summary for HAVCR2

CD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.[supplied by OMIM]

UniProt Comments for HAVCR2

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Disease: Rheumatoid Arthritis

Research Articles on HAVCR2

1. expression is increased on Vdelta T cells of pregnant women
2. Mucin domain-containing molecule 3 (Tim-3) is preferentially expressed in lymphoma-derived endothelial cells.
3. Structural and biologic studies reveal that TIM-3 is a receptor for phosphatidylserine (PtdSer) and that TIM-3 polymorphic variants differ functionally in their recognition of PtdSer and clearance of apoptotic cells.
4. The TIM-3 pathway and the therapeutic potential of modulating the pathway in systemic lupus erythematosus, is reviewed.
5. haplotypes including TIM-3 genetic variants confer susceptibility to asthma in a Chinese population
6. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
7. Tim-3 expression correlates with a dysfunctional and senescent phenotype (CD127(low) CD57(high)), a central rather than effector memory profile (CD45RA(negative) CCR7(high)), and reduced Th1/Tc1 cytokine production.
8. These results suggest that TIM-3 is a negative regulator of human T cells and regulates Th1 and Th17 cytokine secretion.
9. Reduced TIM-3 expression in the lungs of patients may result in a defective T cell ability to control the Th1 immune response and could thus contribute to the pathogenesis of sarcoidosis.
10. The -1516G>T, -574T>G, and 4259G>T of TIM-3 gene polymorphisms might not play an important role as a genetic risk factor in the pathophysiology of ITP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-HAVCR2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>23 publications with HAVCR2 and Neoplasms
Nervous System Diseases Antibodies	>14 publications with HAVCR2 and Nervous System Diseases
Necrosis Antibodies	>10 publications with HAVCR2 and Necrosis
Liver Diseases Antibodies	>9 publications with HAVCR2 and Liver Diseases
Leukemia Antibodies	>6 publications with HAVCR2 and Leukemia
Leukemia, T-Cell Antibodies	>5 publications with HAVCR2 and Leukemia, T-Cell
Arthritis, Rheumatoid Antibodies	>4 publications with HAVCR2 and Arthritis, Rheumatoid
Leukemia, Myeloid, Acute Antibodies	>3 publications with HAVCR2 and Leukemia, Myeloid, Acute
Leukemia, Myeloid Antibodies	>3 publications with HAVCR2 and Leukemia, Myeloid
HTLV-I Infections Antibodies	>3 publications with HAVCR2 and HTLV-I Infections

Organs/Tissues associated with anti-HAVCR2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>101 publications with HAVCR2 and Blood
Liver Antibodies	>10 publications with HAVCR2 and Liver
Spleen Antibodies	>9 publications with HAVCR2 and Spleen
Heart Antibodies	>7 publications with HAVCR2 and Heart
Lung Antibodies	>6 publications with HAVCR2 and Lung
Kidney Antibodies	>5 publications with HAVCR2 and Kidney
Bone Marrow Antibodies	>3 publications with HAVCR2 and Bone Marrow
Pancreas Antibodies	>2 publications with HAVCR2 and Pancreas
Brain Antibodies	>2 publications with HAVCR2 and Brain
Muscle Antibodies	>2 publications with HAVCR2 and Muscle