Mouse Anti-ADAMTS1 Monoclonal Antibody – Cat# MBS606917

Western Blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with MBS606917 (2ug/ml) followed by HRP-conjugated anti-mouse IgG secondary antibody. A specific band was detected for ADAMTS1 at ~60-70kD (as indicated). This experiment was conducted under non-reducing conditions and using Blotting Buffer: 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20; Blocking Solution: 5% nonfat dry milk in Blotting Buffer, Adjust to pH 7.4; Antibody Solution: 2% nonfat dry milk in Blotting Buffer, Adjust to pH 7.4.

ADAMTS1 Antibody – Mouse Monoclonal

Name: ADAMTS1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS606917
Price: 985 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS606917

MBS606917 is an antibody targeting ADAMTS1. Also known as ADAMTS1 (A Disintegrin And Metalloproteinase with ThromboSpondin-1 motif, METH-1). Reactive in Human. Host: Mouse. More details

$985 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ADAMTS1, Monoclonal Antibody

Full Product Name

ADAMTS1 (A Disintegrin And Metalloproteinase with ThromboSpondin-1 motif, METH-1)

Product Gene Name

anti-ADAMTS1 antibody

Product Synonym Names

Anti -ADAMTS1 (A Disintegrin And Metalloproteinase with ThromboSpondin-1 motif, METH-1)

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

9H39

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human ADAMTS1.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

105,354 Da

Chromosome Location

Chromosome: 21; NC_000021.8 (28208606..28217728, complement). Location: 21q21.2

3D Structure

ModBase 3D Structure for Q5HYL0

Immunogen

NS0-derived, recombinant human ADAMTS1 aa253-724.

Product Categories/Family for anti-ADAMTS1 antibody

Antibodies; Abs to Matrix Metalloproteinases

Applications Tested/Suitable for anti-ADAMTS1 antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-ADAMTS1 antibody

Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Immunoprecipitation: 25ug/ml

Other Notes

Small volumes of anti-ADAMTS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADAMTS1. It may not necessarily be applicable to this product.

NCBI GI #

50845384

NCBI GeneID

9510

NCBI Accession #

NP_008919.3

UniProt Primary Accession #

Q5HYL0

UniProt Related Accession #

Q5HYL0; Q6ZP79; Q8NE26; Q9UHI8

GenBank Nucleotide #

NM_006988.3

NCBI Official Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 1 preproprotein

NCBI Official Synonym Full Names

ADAM metallopeptidase with thrombospondin type 1 motif, 1

NCBI Official Symbol

ADAMTS1

NCBI Official Synonym Symbols

C3-C5; METH1; KIAA1346

UniProt Protein Name

Putative uncharacterized protein DKFZp686E01144

Protein Family

A disintegrin and metalloproteinase with thrombospondin motifs

UniProt Gene Name

DKFZp686E01144

UniProt Entry Name

Q5HYL0_HUMAN

NCBI Protein Information

A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; OTTHUMP00000096354; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1

NCBI Summary for ADAMTS1

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq]

UniProt Comments for ADAMTS1

ADAMTS1: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.

Protein type: Secreted; Protease; EC 3.4.24.-; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q21.2

Cellular Component: cytoplasm; basement membrane; cytoplasmic vesicle

Molecular Function: heparin binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: integrin-mediated signaling pathway; negative regulation of cell proliferation; proteolysis; ovulation from ovarian follicle; kidney development

Research Articles on ADAMTS1

1. AHR, a novel acute hypoxia-response sequence, drives reporter gene expression under hypoxia in vitro and in vivo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADAMTS1 antibody

Reference Product	PubMed Publications
FURIN antibody	>8 publications with ADAMTS1 and FURIN
VCAN antibody	>5 publications with ADAMTS1 and VCAN
VEGFA antibody	>3 publications with ADAMTS1 and VEGFA
ACAN antibody	>2 publications with ADAMTS1 and ACAN

Pathways associated with anti-ADAMTS1 antibody

Products by Pathway	Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram

Diseases associated with anti-ADAMTS1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>50 publications with ADAMTS1 and Neoplasms
Cardiovascular Diseases Antibodies	>22 publications with ADAMTS1 and Cardiovascular Diseases
Vascular Diseases Antibodies	>21 publications with ADAMTS1 and Vascular Diseases
Metaplasia Antibodies	>17 publications with ADAMTS1 and Metaplasia
Neovascularization, Pathologic Antibodies	>16 publications with ADAMTS1 and Neovascularization, Pathologic
Carcinoma Antibodies	>15 publications with ADAMTS1 and Carcinoma
Inflammation Antibodies	>14 publications with ADAMTS1 and Inflammation
Osteoarthritis Antibodies	>12 publications with ADAMTS1 and Osteoarthritis
Neoplasm Metastasis Antibodies	>10 publications with ADAMTS1 and Neoplasm Metastasis
Neoplasms, Experimental Antibodies	>10 publications with ADAMTS1 and Neoplasms, Experimental

Organs/Tissues associated with anti-ADAMTS1 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>48 publications with ADAMTS1 and Vascular
Ovary Antibodies	>24 publications with ADAMTS1 and Ovary
Bone Antibodies	>20 publications with ADAMTS1 and Bone
Kidney Antibodies	>19 publications with ADAMTS1 and Kidney
Heart Antibodies	>18 publications with ADAMTS1 and Heart
Connective Tissue Antibodies	>18 publications with ADAMTS1 and Connective Tissue
Muscle Antibodies	>18 publications with ADAMTS1 and Muscle
Embryonic Tissue Antibodies	>12 publications with ADAMTS1 and Embryonic Tissue
Brain Antibodies	>12 publications with ADAMTS1 and Brain
Lung Antibodies	>10 publications with ADAMTS1 and Lung