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CEACAM 5,6 (CEACAM5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS607182

CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy- More details

$835 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CEACAM 5,6 (CEACAM5), Monoclonal Antibody
Full Product Name
CEACAM 5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)
Product Gene Name
Product Synonym Names
Anti -CEACAM 5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5E505
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector.
Specificity
MUS reacts specifically with CEACAM5 (CEA/CD66e) and CEACAM6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.
Preparation and Storage
-20 degree C
Molecular Weight
76,795 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (42212530..42234437). Location: 19q13.1-q13.2
OMIM
114890
3D Structure
ModBase 3D Structure for P06731
Immunogen
Immunization of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
Applications Tested/Suitable for anti-CEACAM5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CEACAM5 antibody
MUS has been tested for the use in flow cytometry, cell-ELISA, ELISA, Western blots and immunohistology on cryosections.

Testing Data of anti-CEACAM5 antibody

Fig. 1: Specificity Testing: BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6; CEACAM7; CEACAM8; PSG; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified antibody was tested on all CEACAM|transfectants. A positive signal was obtained with CEACAM5 and CEACAM6|expressing cells (red curves).
Mouse Anti-CEACAM5 Monoclonal Antibody – Cat# MBS607182

Testing Data of anti-CEACAM5 antibody

Fig. 2: Domain organization of the human CEACAM family. The CEACAM family consists of two subgroups, the CEACAM and the PSG subgroup. Old names appear below the consensus nomenclature. CEACAM family members are membrane-bound by either via a transmembrane domain or a GPI anchor (green arrow) whereas the PSGs are secreted glycoproteins. N-linked glycosylation sites are shown as drum sticks. The IgV domains are shown in red, the IgC domains in blue.
Mouse Anti-CEACAM5 Monoclonal Antibody, Testing Data – Cat# MBS607182

Other Notes

Small volumes of anti-CEACAM5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CEACAM5 purchase

MBS607182 is an antibody targeting CEACAM5. Also known as CEACAM 5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CEACAM5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Synonym Protein Names
Carcinoembryonic antigen; CEA; Meconium antigen 100
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100

NCBI Summary for CEACAM5

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]

UniProt Comments for CEACAM5

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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