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Radical Fringe Homolog (RFNG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS607259

The Drosophila melanogaster 'fringe' gene is a secreted signaling protein that is involved in the formation of boundaries between groups of cells during development. It acts by modulating the activation of te Notch signal transduction pathway. A human radical… More details

$750 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Radical fringe homolog (RFNG), Monoclonal Antibody
Full Product Name
Radical fringe homolog (Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, RFNG)
Product Gene Name
Product Synonym Names
Anti -Radical fringe homolog (Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, RFNG)
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G432
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Specificity
Recognizes human radical fringe homolog.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
20,903 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (80005778..80009650, complement). Location: 17q25
3D Structure
ModBase 3D Structure for Q8N9R1
Immunogen
Partial recombinant human radical fringe homolog (aa222-330) with a GST tag.
Applications Tested/Suitable for anti-RFNG antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-RFNG antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10 ug/ml

Immunofluorescence (IF) of anti-RFNG antibody

Immunofluorescent detection of Radical fringe homolog expression in HepG2 cells. The anti-Radical fringe homolog primary antibody was used at a concentration of 10 ug/ml.
Mouse Anti-RFNG Monoclonal Antibody – Cat# MBS607259

Western Blot (WB) of anti-RFNG antibody

Western blot detection Radical fringe homolog expression in HepG2 cells.
Mouse Anti-RFNG Monoclonal Antibody, Western Blot (WB) – Cat# MBS607259

Other Notes

Small volumes of anti-RFNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFNG purchase

MBS607259 is an antibody targeting RFNG. Also known as Radical fringe homolog (Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, RFNG). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RFNG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
radical fringe homolog (Drosophila), isoform CRA_d
NCBI Official Synonym Full Names
RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol
RFNG
NCBI Official Synonym Symbols
RFNG
UniProt Protein Name
cDNA FLJ36697 fis, clone UTERU2008962, moderately similar to Homo sapiens radical fringe (RFNG) gene
UniProt Entry Name
Q8N9R1_HUMAN
NCBI Protein Information
beta-1,3-N-acetylglucosaminyltransferase radical fringe; radical fringe homolog; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

UniProt Comments for RFNG

RFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis. Belongs to the glycosyltransferase 31 family.

Protein type: Membrane protein, integral; Transferase; EC 2.4.1.222; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular region; integral to Golgi membrane

Molecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity

Biological Process: nervous system development; organ morphogenesis; positive regulation of protein binding; metabolic process; cell differentiation; pattern specification process; positive regulation of Notch signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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