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BUBR1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS607457

MBS607457 is an antibody targeting BUBR1. Also known as BubR1 (Budding Uninhibited by Benzimidazoles Related 1, BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog beta, Budding Uninhibited by Benzimidazoles 1 beta, BUB1beta, BUB1B, Bub1A, hBUBR1,… More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BubR1, Monoclonal Antibody
Full Product Name
BubR1 (Budding Uninhibited by Benzimidazoles Related 1, BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog beta, Budding Uninhibited by Benzimidazoles 1 beta, BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3/BUB1-related Protein Kinase, MAD3L, Mitotic Checkpoint Ge
Product Gene Name
anti-BUBR1 antibody
Product Synonym Names
Anti -BubR1 (Budding Uninhibited by Benzimidazoles Related 1, BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog beta, Budding Uninhibited by Benzimidazoles 1 beta, BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3/BUB1-related Protein Kinase, MAD3L, Mitotic Checkpoint Ge
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9j24
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human BUB1B.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
45,374 Da
Chromosome Location
Chromosome: 2; NC_003071.7 (14213601..14215999, complement). Location: chromosome: 2
3D Structure
ModBase 3D Structure for O22806
Immunogen
Synthetic peptide corresponding to the middle region of human BUB1B

Product Categories/Family for anti-BUBR1 antibody

Antibodies; Abs to DNA Replication; Repair
Applications Tested/Suitable for anti-BUBR1 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-BUBR1 antibody
Suitable for use in Western Blot and Immunocytochemistry.

Testing Data of anti-BUBR1 antibody

Mouse Anti-BUBR1 Monoclonal Antibody – Cat# MBS607457

Immunostaining (IS) of anti-BUBR1 antibody

IC: Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BUB1B mAb.
Mouse Anti-BUBR1 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS607457

Other Notes

Small volumes of anti-BUBR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BUBR1. It may not necessarily be applicable to this product.

NCBI GI #
334184662
NCBI GeneID
817921
NCBI Accession #
NP_001189668.1
UniProt Primary Accession #
O22806
UniProt Secondary Accession #
Q8L9V1; F4IFV2
UniProt Related Accession #
O22806
GenBank Nucleotide #
NM_001202739.1
NCBI Official Full Name
BUB1-related protein BUBR1
NCBI Official Symbol
BUBR1
NCBI Official Synonym Symbols
BUB1-related (BUB1: budding uninhibited by benzymidazol 1); BUBR1; F4P9.33; F4P9_33
UniProt Protein Name
Mitotic spindle checkpoint protein BUBR1
UniProt Synonym Protein Names
BUB1-related protein 1
Protein Family
Mitotic spindle checkpoint protein
UniProt Gene Name
BUBR1
UniProt Synonym Gene Names
MAD3
UniProt Entry Name
BUBR1_ARATH
NCBI Protein Information
BUB1-related protein BUBR1

NCBI Summary for BUBR1

Encodes BUBR1. May have the spindle assembly checkpoint protein functions conserved from yeast to humans.

UniProt Comments for BUBR1

Function: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions may be to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C

By similarity. Ref.5

Subunit structure: Interacts with anaphase-promoting complex/cyclosome (APC/C)

By similarity. Part of the mitotic checkpoint complex (MCC); interacts with CDC20-1 and CDC20-2. Interacts with MAD2 at chromocenters and with BUB3.1. Ref.5 Ref.6

Subcellular location: Chromosome. Cytoplasm. Nucleus. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

By similarity. Note: Cytoplasmic in interphase cells. Accumulates onto both kinetochores and the spindle microtubules in cell arrested in metaphase. Associates with unattached kinetochores in early prophase upon spindle assembly checkpoint (SAC) activation. Present in chromocenters. Ref.5

Tissue specificity: Expressed in actively dividing tissues, early in organ development, in young leaves, lateral root primordia and root meristems. Ref.5

Induction: Cell cycle regulated expression with a distinct expression peak at the G2/M boundary. Ref.5

Domain: The KEN box is required for its ubiquitination and degradation

By similarity.BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.

Sequence similarities: Contains 1 BUB1 N-terminal domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BUBR1 antibody

 Reference Product  PubMed Publications
 AT3G25980 antibody  >1 publications with BUBR1 and AT3G25980

Organs/Tissues associated with anti-BUBR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Flower Antibodies  >1 publications with BUBR1 and Flower
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