Mouse Anti-CRMP1 Monoclonal Antibody – Cat# MBS607826

CL-1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS607826 (1:2000). Proteins were visualized using a goat anti-mouse IgM secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates CRMP1 (60-65kD).

Collapsin Response Mediator Protein 1 (CRMP1) Antibody – Mouse Monoclonal

Name: CRMP1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS607826
Price: 1015 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS607826

Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis. Their continued expression in areas of high synaptic remodeling such as the… More details

$1015 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Collapsin Response Mediator Protein 1 (CRMP1), Monoclonal Antibody

Full Product Name

Collapsin Response Mediator Protein 1 (CRMP1, Dihydropyrimidinase-like 1, DPYSL1, DRP1, ULIP3, CRAM)

Product Gene Name

anti-CRMP1 antibody

Product Synonym Names

Anti -Collapsin Response Mediator Protein 1 (CRMP1, Dihydropyrimidinase-like 1, DPYSL1, DRP1, ULIP3, CRAM)

Clonality

Monoclonal

Isotype

IgM

Clone Number

5H23

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid with 0.05% sodium azide before the addition of glycerol to 30%.

Purity/Purification

Ascites
Ascites

Specificity

Recognizes CRMP1, Mr ~60-65kD. Additional non-specific bands of 50-55kD detected in long exposures. Species Crossreactivity: Human.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

62,184 Da

Chromosome Location

chromosome: 4; Location: 4p16.1

OMIM

602462

3D Structure

ModBase 3D Structure for Q14194

Immunogen

His6-tagged fusion protein corresponding to aa76-572 of human CRMP1.

Product Categories/Family for anti-CRMP1 antibody

Antibodies; Abs to Neuroscience

Applications Tested/Suitable for anti-CRMP1 antibody

Western Blot (WB)

Application Notes for anti-CRMP1 antibody

Suitable for use in Western Blot.
Dilution: Immunoblot Analysis: A 1:500-1:2000 dilution of this lot detected CRMP1 in lung adenocarcinoma CL-1 and HeLa whole cell lysates.

Other Notes

Small volumes of anti-CRMP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRMP1 purchase

MBS607826 is an antibody targeting CRMP1. Also known as Collapsin Response Mediator Protein 1 (CRMP1, Dihydropyrimidinase-like 1, DPYSL1, DRP1, ULIP3, CRAM). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CRMP1. It may not necessarily be applicable to this product.

NCBI GI #

14189905

NCBI GeneID

1400

UniProt Primary Accession #

Q14194

UniProt Secondary Accession #

Q13024; Q4W5F1; Q96TC8

UniProt Related Accession #

Q14194; Q96I11; A0EJG6

NCBI Official Full Name

collapsin response mediator protein 1

NCBI Official Synonym Full Names

collapsin response mediator protein 1

NCBI Official Symbol

CRMP1

NCBI Official Synonym Symbols

DRP1; DRP-1; CRMP-1; DPYSL1; ULIP-3; CRMP1

UniProt Protein Name

Dihydropyrimidinase-related protein 1

UniProt Synonym Protein Names

Collapsin response mediator protein 1; CRMP-1; Unc-33-like phosphoprotein 3

Protein Family

Dihydropyrimidinase-related protein

UniProt Gene Name

CRMP1

UniProt Synonym Gene Names

DPYSL1; ULIP3

UniProt Entry Name

DPYL1_HUMAN

NCBI Protein Information

dihydropyrimidinase-related protein 1; OTTHUMP00000217352; dihydropyrimidinase-like 1; unc-33-like phosphoprotein 3; dihydropyrimidinase related protein-1

NCBI Summary for CRMP1

This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

UniProt Comments for CRMP1

CRMP-1: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Hydrolase; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: cell soma; dendrite; cytoplasm; microtubule organizing center; spindle; cytosol

Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides; protein binding

Biological Process: axon guidance; nervous system development; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine base catabolic process

Research Articles on CRMP1

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
2. Observational study of gene-disease association. (HuGE Navigator)
3. loss of CRMP1 contribute to the increased invasive phenotype of human Glioblastoma multiforme brain tumors expressing mutant EGFRvIII.
4. Results suggest that Drp1 appears to affect the activity of the mitochondrial fusion machinery by unbalancing the protein levels of mitofusins and OPA1.
5. Drp1 is dephosphorylated in PINK1 deficient cells due to activation of the calcium-dependent phosphatase calcineurin.
6. ChIP and EMSA demonstrated that p50 binds to a kappaB site residing between -1753 and -1743 of the CRMP-1 promoter region.
7. Transcription of the invasion suppressor, CRMP-1, is reciprocally regulated at the promoter region by C/EBPalpha and Sp1.
8. CTGF inhibits metastasis and invasion of human lung adenocarcinoma by a CRMP-1-dependent mechanism.
9. The CRMP1 protein is characterizing as modulators of Wnt protein signaling of Axin-1.
10. CRMP1 and EVC genes are located near WFS1, the Wolfram syndrome type 1 gene.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CRMP1 antibody

Reference Product	PubMed Publications
AXIN1 antibody	>1 publications with CRMP1 and AXIN1

Pathways associated with anti-CRMP1 antibody

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
CRMPs In Sema3A Signaling Pathway antibodies	CRMPs In Sema3A Signaling Pathway Diagram
Semaphorin Interactions Pathway antibodies	Semaphorin Interactions Pathway Diagram

Diseases associated with anti-CRMP1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>13 publications with CRMP1 and Neoplasms
Neurodegenerative Diseases Antibodies	>5 publications with CRMP1 and Neurodegenerative Diseases
Neoplasm Metastasis Antibodies	>3 publications with CRMP1 and Neoplasm Metastasis
Lung Neoplasms Antibodies	>3 publications with CRMP1 and Lung Neoplasms
Adenoma Antibodies	>2 publications with CRMP1 and Adenoma
Necrosis Antibodies	>2 publications with CRMP1 and Necrosis
Neurologic Manifestations Antibodies	>2 publications with CRMP1 and Neurologic Manifestations
Inflammation Antibodies	>2 publications with CRMP1 and Inflammation
Pain Antibodies	>1 publications with CRMP1 and Pain
Liver Cirrhosis Antibodies	>1 publications with CRMP1 and Liver Cirrhosis

Organs/Tissues associated with anti-CRMP1 antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>39 publications with CRMP1 and Nerve
Brain Antibodies	>18 publications with CRMP1 and Brain
Embryonic Tissue Antibodies	>7 publications with CRMP1 and Embryonic Tissue
Muscle Antibodies	>5 publications with CRMP1 and Muscle
Lung Antibodies	>4 publications with CRMP1 and Lung
Heart Antibodies	>1 publications with CRMP1 and Heart
Intestine Antibodies	>1 publications with CRMP1 and Intestine
Testis Antibodies	>1 publications with CRMP1 and Testis
Uncharacterized Tissue Antibodies	>1 publications with CRMP1 and Uncharacterized Tissue
Eye Antibodies	>1 publications with CRMP1 and Eye