Neurofilament, Heavy Chain, 180-220kD Antibody – Mouse Monoclonal

Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220kD with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M, this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly, in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury.

Can be used on cryostat sections and on formalin-fixed cells in tissue culture. The antibody works well in ELISA and Western Blot, and is particularly appropriate for paraffin-embedded histological sections.

Small volumes of anti-Neurofilament, heavy chain, 180-220kD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS607916 is an antibody targeting Neurofilament, heavy chain, 180-220kD. Also known as Neurofilament, heavy chain, 180-220kD (NF-H). Reactive in Avian, Human, Porcine. Host: Mouse.