Mouse Anti-GZMK Monoclonal Antibody – Cat# MBS607923

WB: NIH 3T3 cell lysate probed with MBS607923 at 0.1ug/ml.

GRK 2,3 (GZMK) Antibody – Mouse Monoclonal

Name: GZMK Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS607923
Price: 1045 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS607923

MBS607923 is an antibody targeting GZMK. Also known as GRK 2,3 (betaARK 1,2). Reactive in Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat. Host: Mouse. More details

$1045 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GRK 2,3 (GZMK), Monoclonal Antibody

Full Product Name

GRK 2,3 (betaARK 1,2)

Product Gene Name

anti-GZMK antibody

Product Synonym Names

Anti -GRK 2,3 (betaARK 1,2)

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

0.T.54

Host

Mouse

Species Reactivity

Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat

Form/Format

Supplied in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide., 40% glycerol.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human GRK2/3 at ~80 kD. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody. Epitope maps to aa483-485. Species Crossreactivity: rat, mouse, chicken, bovine, porcine and rabbit.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

28,882 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (54320107..54329960). Location: 5q11.2

OMIM

600784

3D Structure

ModBase 3D Structure for P49863

Immunogen

Partial GST fusion protein corresponding to the GRK3 (bARK2) carboxyl terminus (aa 467-688). This immunogen shares homology with GRK2 (bARK1). The minimal epitope recognized by the antibody is aa 483-485.

Product Categories/Family for anti-GZMK antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-GZMK antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-GZMK antibody

Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Immunoprecipitation: This antibody can be used to immunoprecipitate GRK2/3 from transfected cells.
Western Blot: 0.1-1ug/ml. Detects GRK2/3 in RIPA lysates from mouse 3T3 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8972 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Other Notes

Small volumes of anti-GZMK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMK. It may not necessarily be applicable to this product.

NCBI GI #

4504235

NCBI GeneID

3003

NCBI Accession #

NP_002095.1

UniProt Primary Accession #

P49863

UniProt Secondary Accession #

B2R563

UniProt Related Accession #

P49863

GenBank Nucleotide #

NM_002104.2

NCBI Official Full Name

granzyme K

NCBI Official Synonym Full Names

granzyme K (granzyme 3; tryptase II)

NCBI Official Symbol

GZMK

NCBI Official Synonym Symbols

TRYP2

UniProt Protein Name

Granzyme K

UniProt Synonym Protein Names

Fragmentin-3; Granzyme-3; NK-tryptase-2

Protein Family

Granzyme

UniProt Gene Name

GZMK

UniProt Synonym Gene Names

TRYP2; NK-Tryp-2

UniProt Entry Name

GRAK_HUMAN

NCBI Protein Information

granzyme K; NK-Tryp-2; granzyme 3; granzyme-3; tryptase II; fragmentin-3; NK-tryptase-2; granzyme K (serine protease, granzyme 3; tryptase II)

NCBI Summary for GZMK

This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

UniProt Comments for GZMK

GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis

Research Articles on GZMK

1. Granzyme K plays an important physiological role in immunoregulation of adaptive immunity underlying the cytotoxicity of CD56(bright) natural killer (NK) cells toward activated T cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GZMK antibody

Reference Product	PubMed Publications
GZMB antibody	>11 publications with GZMK and GZMB
GZMA antibody	>11 publications with GZMK and GZMA
PRF1 antibody	>8 publications with GZMK and PRF1
CCR5 antibody	>1 publications with GZMK and CCR5
GZMH antibody	>1 publications with GZMK and GZMH
CD2 antibody	>1 publications with GZMK and CD2

Diseases associated with anti-GZMK antibody

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>4 publications with GZMK and Immune System Diseases
Inflammation Antibodies	>4 publications with GZMK and Inflammation
Heart Diseases Antibodies	>1 publications with GZMK and Heart Diseases
Depressive Disorder Antibodies	>1 publications with GZMK and Depressive Disorder
Necrosis Antibodies	>1 publications with GZMK and Necrosis

Organs/Tissues associated with anti-GZMK antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>24 publications with GZMK and Blood
Lung Antibodies	>3 publications with GZMK and Lung
Brain Antibodies	>1 publications with GZMK and Brain
Thymus Antibodies	>1 publications with GZMK and Thymus