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Transglutaminase II Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS608109

Transglutaminase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. It is also known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Transglutaminase II, Monoclonal Antibody
Full Product Name
Transglutaminase II (BSA & Azide Free)
Product Synonym Names
Anti -Transglutaminase II (BSA & Azide Free)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F382
Host
Mouse
Species Reactivity
Canine, Guinea Pig, Human, Rabbit
Form/Format
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T8260-04
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes guinea pig Transglutaminase II. Species Crossreactivity: human, canine and rabbit Does not react with mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
77-85kD
Immunogen
Puri?ed guinea pig liver TGase II. MW of Antigen: 77-85kD. Epitope: aa447-478. Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix
Positive Control
HUVEC cells. Placenta
Applications Tested/Suitable for anti-Transglutaminase II antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Transglutaminase II antibody
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Affinity Purification, Biological Blockade.
Dilution: Western Blot: 1-2ug/ml for 2 hours ar RT
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by researcher.

Western Blot (WB) of anti-Transglutaminase II antibody

Western Blot using MBS608109.
Mouse Anti-Transglutaminase II Monoclonal Antibody – Cat# MBS608109

Western Blot (WB) of anti-Transglutaminase II antibody

IP/Western Blot using MBS608109.
Mouse Anti-Transglutaminase II Monoclonal Antibody, Western Blot (WB) – Cat# MBS608109

Immunohistochemistry (IHC) of anti-Transglutaminase II antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MBS608109 using peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining of tumor and endothelial cells.
Mouse Anti-Transglutaminase II Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS608109

Other Notes

Small volumes of anti-Transglutaminase II antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Transglutaminase II purchase

MBS608109 is an antibody targeting Transglutaminase II. Also known as Transglutaminase II (BSA & Azide Free). Reactive in Canine, Guinea Pig, Human, Rabbit. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Transglutaminase II. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
transglutaminase II

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Transglutaminase II antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Transglutaminase II and FLNA
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