ROK Alpha/ROCK-II (ROCK2) Antibody – Rabbit Monoclonal

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; DMPK family; ROCK subfamily

Cellular Component: centrosome; cytoplasm; plasma membrane; microtubule organizing center; spindle pole centrosome; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; kinase activity; ATP binding

Biological Process: chromosome condensation; negative regulation of angiogenesis; smooth muscle contraction; induction of apoptosis via death domain receptors; regulation of actin cytoskeleton organization and biogenesis; dendrite morphogenesis; neural tube closure; rhythmic process; regulation of circadian rhythm; actin cytoskeleton organization and biogenesis; centrosome duplication; protein amino acid phosphorylation; regulation of keratinocyte differentiation; Rho protein signal transduction