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egr1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS608553

EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors,… More details

$820 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EGR1, Monoclonal Antibody
Full Product Name
EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)
Product Gene Name
Product Synonym Names
Anti -EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)
Clonality
Monoclonal
Isotype
IgG
Clone Number
9j16
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity/Purification
Supernatant
Supernatant
Specificity
Recognizes endogenous levels of total human EGR1 at ~75kD. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Molecular Weight
55,140 Da
Chromosome Location
Chromosome: 14; NC_007125.5 (22334987..22337228, complement)
3D Structure
ModBase 3D Structure for P26632
Immunogen
Synthetic peptide corresponding to the amino-terminal sequence of human EGR1.
Applications Tested/Suitable for anti-egr1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-egr1 antibody
Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunofluorescence (IF-IC): 1:800
Immunohistochemistry (Paraffin): 1:100
Immunoprecipitation: 1:50
Chromatin Immunoprecipitation: 1:50

Western Blot (WB) of anti-egr1 antibody

WB analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours) using MBS608553.
Rabbit Anti-egr1 Monoclonal Antibody – Cat# MBS608553

Immunoprecipitation (IP) of anti-egr1 antibody

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 THP-1 cells treated with TPA (30ng/ml) overnight and either 10ul of MBS608553 or 2ul of Normal Rabbit IgG. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Rabbit Anti-egr1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS608553

Immunofluorescence (IF) of anti-egr1 antibody

Confocal immunofluorescent analysis analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right), using MBS608553 (green) and MEK1/2 mouse mAb (red).
Rabbit Anti-egr1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS608553

Immunohistochemistry (IHC) of anti-egr1 antibody

Immunohistochemical staining of human non-Hodgkin’s lymphoma using MBS608553.
Rabbit Anti-egr1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS608553

Immunohistochemistry (IHC) of anti-egr1 antibody

Immunohistochemical analysis of paraffin-embedded mouse brain, using MBS608553.
Rabbit Anti-egr1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS608553

Other Notes

Small volumes of anti-egr1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for egr1 purchase

MBS608553 is an antibody targeting egr1. Also known as EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for egr1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
early growth response protein 1
NCBI Official Synonym Full Names
early growth response 1
NCBI Official Symbol
egr1
NCBI Official Synonym Symbols
krox24; krox-24; wu:fj64b05; wu:fq25f01
UniProt Protein Name
Early growth response protein 1
UniProt Synonym Protein Names
Zinc finger protein Krox-24
UniProt Gene Name
egr1
UniProt Synonym Gene Names
krox24
UniProt Entry Name
EGR1_DANRE
NCBI Protein Information
early growth response protein 1; EGR-1; etID309970.14; zinc finger protein Krox-24

UniProt Comments for egr1

Function: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation

Subcellular location: Nucleus.

Induction: By growth factors.

Sequence similarities: Belongs to the EGR C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-egr1 antibody

 Products by Pathway  Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
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