Mouse Anti-IRS1 Monoclonal Antibody – Cat# MBS609061

Indirect ELISA Serial dilution of the antibody starting with 5ug/well was done to check for the affinity. 1 μg of coated antigen can be sensitively detected by anti-human IRS Ser 616-Phospho antibody used at 300 pg/well concentration.

IRS-1 (IRS1) Antibody – Mouse Monoclonal

Name: IRS1 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS609061
Price: 630 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609061

Insulin receptor substrates (IRS) subtypes are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. The balance between Ser/Thr phosphorylation of IRS has… More details

$630 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IRS-1 (IRS1), Monoclonal Antibody

Full Product Name

IRS-1, phosphorylated (Ser616) (Insulin Receptor Substrate 1, IRS1)

Product Gene Name

anti-IRS1 antibody

Product Synonym Names

Anti -IRS-1, phosphorylated (Ser616) (Insulin Receptor Substrate 1, IRS1)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

10g79

Host

Mouse

Form/Format

Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Phospho-IRS (Ser616).

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

131,591 Da

Chromosome Location

Chromosome: 2; NC_000002.11 (227596033..227663506, complement). Location: 2q36

OMIM

125853

3D Structure

ModBase 3D Structure for P35568

Immunogen

Synthetic peptide corresponding to IRS-1, phosphorylated at Ser616 site.

Product Categories/Family for anti-IRS1 antibody

Antibodies; Abs to Protein Kinase Substrates

Applications Tested/Suitable for anti-IRS1 antibody

ELISA (EL/EIA)

Application Notes for anti-IRS1 antibody

Suitable for use in Indirect ELISA.
Dilution: Indirect ELISA: 1-2ug/ml

Other Notes

Small volumes of anti-IRS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRS1 purchase

MBS609061 is an antibody targeting IRS1. Also known as IRS-1, phosphorylated (Ser616) (Insulin Receptor Substrate 1, IRS1). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IRS1. It may not necessarily be applicable to this product.

NCBI GI #

5031805

NCBI GeneID

3667

NCBI Accession #

NP_005535.1

UniProt Primary Accession #

P35568

UniProt Related Accession #

P35568

GenBank Nucleotide #

NM_005544.2

NCBI Official Full Name

insulin receptor substrate 1

NCBI Official Synonym Full Names

insulin receptor substrate 1

NCBI Official Symbol

IRS1

NCBI Official Synonym Symbols

HIRS-1

UniProt Protein Name

Insulin receptor substrate 1

Protein Family

Isoleucine--tRNA ligase

UniProt Gene Name

IRS1

UniProt Entry Name

IRS1_HUMAN

NCBI Protein Information

insulin receptor substrate 1; IRS-1; OTTHUMP00000164234

NCBI Summary for IRS1

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]

UniProt Comments for IRS1

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; glucose homeostasis; signal transduction; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent

Research Articles on IRS1

1. miR-126 was actively involved in the development of insulin resistance induced by mitochondrial dysfunction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRS1 antibody

Reference Product	PubMed Publications
IGF1R antibody	>212 publications with IRS1 and IGF1R
AKT1 antibody	>165 publications with IRS1 and AKT1
GRB2 antibody	>136 publications with IRS1 and GRB2
MTOR antibody	>107 publications with IRS1 and MTOR
MAPK1 antibody	>73 publications with IRS1 and MAPK1
SHC1 antibody	>64 publications with IRS1 and SHC1
JAK2 antibody	>55 publications with IRS1 and JAK2
PTPN11 antibody	>41 publications with IRS1 and PTPN11
INSR antibody	>32 publications with IRS1 and INSR
SOCS3 antibody	>30 publications with IRS1 and SOCS3

Pathways associated with anti-IRS1 antibody

Products by Pathway	Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Aldosterone-regulated Sodium Reabsorption Pathway antibodies	Aldosterone-regulated Sodium Reabsorption Pathway Diagram
Aldosterone-regulated Sodium Reabsorption Pathway antibodies	Aldosterone-regulated Sodium Reabsorption Pathway Diagram
Alpha6-Beta4 Integrin Signaling Pathway antibodies	Alpha6-Beta4 Integrin Signaling Pathway Diagram
Downstream Signaling Of Activated FGFR Pathway antibodies	Downstream Signaling Of Activated FGFR Pathway Diagram
EPO Receptor Signaling Pathway antibodies	EPO Receptor Signaling Pathway Diagram
IGF1 Pathway antibodies	IGF1 Pathway Diagram
IL-2 Signaling Pathway antibodies	IL-2 Signaling Pathway Diagram

Diseases associated with anti-IRS1 antibody

Disease Name	Pubmed Publications
Insulin Resistance Antibodies	>920 publications with IRS1 and Insulin Resistance
Hyperinsulinism Antibodies	>622 publications with IRS1 and Hyperinsulinism
Neoplasms Antibodies	>310 publications with IRS1 and Neoplasms
Obesity Antibodies	>286 publications with IRS1 and Obesity
Diabetes Mellitus, Type 2 Antibodies	>254 publications with IRS1 and Diabetes Mellitus, Type 2
Necrosis Antibodies	>132 publications with IRS1 and Necrosis
Breast Neoplasms Antibodies	>78 publications with IRS1 and Breast Neoplasms
Inflammation Antibodies	>64 publications with IRS1 and Inflammation
Hypertension Antibodies	>64 publications with IRS1 and Hypertension
Fatty Liver Antibodies	>63 publications with IRS1 and Fatty Liver

Organs/Tissues associated with anti-IRS1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>698 publications with IRS1 and Muscle
Liver Antibodies	>362 publications with IRS1 and Liver
Connective Tissue Antibodies	>200 publications with IRS1 and Connective Tissue
Pancreas Antibodies	>124 publications with IRS1 and Pancreas
Ovary Antibodies	>98 publications with IRS1 and Ovary
Brain Antibodies	>84 publications with IRS1 and Brain
Heart Antibodies	>83 publications with IRS1 and Heart
Kidney Antibodies	>82 publications with IRS1 and Kidney
Nerve Antibodies	>37 publications with IRS1 and Nerve
Bone Antibodies	>33 publications with IRS1 and Bone