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IRS1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609261

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2… More details

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Product Overview

Online Datasheet in PDF
Product Name
IRS1, Monoclonal Antibody
Full Product Name
IRS1, phosphorylated (Ser307 mouse/ Ser312 human) (insulin receptor substrate 1)
Product Gene Name
anti-IRS1 antibody
Product Synonym Names
Anti -IRS1, phosphorylated (Ser307 mouse/ Ser312 human) (insulin receptor substrate 1)
Clonality
Monoclonal
Isotype
IgG2ak
Clone Number
9H250
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human). Species Crossreactivity: rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
131,591 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (227596033..227663506, complement). Location: 2q36
OMIM
125853
3D Structure
ModBase 3D Structure for P35568
Immunogen
Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.

Product Categories/Family for anti-IRS1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-IRS1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-IRS1 antibody
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, and Immunocytochemistry.
Dilution: Western Blot: 1:1000
Immunocytochemistry: 1:500
Immunoprecipitation: 1:1000

Western Blot (WB) of anti-IRS1 antibody

Western Blot Analysis:|Untreated (lanes 1, 3, 5) and Calyculin A/Okadaic Acid-treated (lanes 2, 4, 6) IR/IRS1 transfected CHO cell lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti-phospho-IRS1 (Ser307) (1:1,000 dilution). |Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrow indicates IRS1 phosphorylated on Ser307 (~185kD).
Mouse Anti-IRS1 Monoclonal Antibody – Cat# MBS609261

Western Blot (WB) of anti-IRS1 antibody

Western Blot Analysis:|Untreated (lanes 1) and Insulin-treated (lanes 2) NIH/3T3-L1 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti-phospho-IRS1 (Ser307) (1:1,000 dilution). |Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrow indicates IRS1 phosphorylated on Ser307 (~185kD).
Mouse Anti-IRS1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS609261

Other Notes

Small volumes of anti-IRS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRS1 purchase

MBS609261 is an antibody targeting IRS1. Also known as IRS1, phosphorylated (Ser307 mouse/ Ser312 human) (insulin receptor substrate 1). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IRS1. It may not necessarily be applicable to this product.

NCBI GI #
5031805
NCBI GeneID
3667
NCBI Accession #
NP_005535.1
UniProt Primary Accession #
P35568
UniProt Related Accession #
P35568
GenBank Nucleotide #
NM_005544.2
NCBI Official Full Name
insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
UniProt Protein Name
Insulin receptor substrate 1
Protein Family
Isoleucine--tRNA ligase
UniProt Gene Name
IRS1
UniProt Entry Name
IRS1_HUMAN
NCBI Protein Information
insulin receptor substrate 1; IRS-1; OTTHUMP00000164234

NCBI Summary for IRS1

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]

UniProt Comments for IRS1

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; signal transduction; glucose homeostasis; negative regulation of insulin secretion; response to insulin stimulus; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent
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Research Articles on IRS1

1. miR-126 was actively involved in the development of insulin resistance induced by mitochondrial dysfunction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRS1 antibody

 Reference Product  PubMed Publications
 IGF1R antibody  >212 publications with IRS1 and IGF1R
 AKT1 antibody  >165 publications with IRS1 and AKT1
 GRB2 antibody  >136 publications with IRS1 and GRB2
 MTOR antibody  >107 publications with IRS1 and MTOR
 MAPK1 antibody  >73 publications with IRS1 and MAPK1
 SHC1 antibody  >64 publications with IRS1 and SHC1
 JAK2 antibody  >55 publications with IRS1 and JAK2
 PTPN11 antibody  >41 publications with IRS1 and PTPN11
 INSR antibody  >32 publications with IRS1 and INSR
 SOCS3 antibody  >30 publications with IRS1 and SOCS3

Pathways associated with anti-IRS1 antibody

 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 IGF1 Pathway antibodies  IGF1 Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram

Diseases associated with anti-IRS1 antibody

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >920 publications with IRS1 and Insulin Resistance
 Hyperinsulinism Antibodies  >622 publications with IRS1 and Hyperinsulinism
 Neoplasms Antibodies  >310 publications with IRS1 and Neoplasms
 Obesity Antibodies  >286 publications with IRS1 and Obesity
 Diabetes Mellitus, Type 2 Antibodies  >254 publications with IRS1 and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >132 publications with IRS1 and Necrosis
 Breast Neoplasms Antibodies  >78 publications with IRS1 and Breast Neoplasms
 Inflammation Antibodies  >64 publications with IRS1 and Inflammation
 Hypertension Antibodies  >64 publications with IRS1 and Hypertension
 Fatty Liver Antibodies  >63 publications with IRS1 and Fatty Liver

Organs/Tissues associated with anti-IRS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >698 publications with IRS1 and Muscle
 Liver Antibodies  >362 publications with IRS1 and Liver
 Connective Tissue Antibodies  >200 publications with IRS1 and Connective Tissue
 Pancreas Antibodies  >124 publications with IRS1 and Pancreas
 Ovary Antibodies  >98 publications with IRS1 and Ovary
 Brain Antibodies  >84 publications with IRS1 and Brain
 Heart Antibodies  >83 publications with IRS1 and Heart
 Kidney Antibodies  >82 publications with IRS1 and Kidney
 Nerve Antibodies  >37 publications with IRS1 and Nerve
 Bone Antibodies  >33 publications with IRS1 and Bone
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