Rat Anti-PRSS3 Monoclonal Antibody – Cat# MBS609301

Trypsin-3 (PRSS3) Antibody – Rat Monoclonal

Name: PRSS3 Antibody; Monoclonal; Rat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS609301
Price: 970 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609301

Trypsin-3, also known as mesotrypsin, is one of the three trypsins produced by the pancreas. It consists of a pro peptide (aa 16 to 23) and a mature chain (aa 24 to 247). More details

$970 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Trypsin-3 (PRSS3), Monoclonal Antibody

Full Product Name

Trypsin-3 (PRSS3, Mesotrypsin)

Product Gene Name

anti-PRSS3 antibody

Product Synonym Names

Anti -Trypsin-3 (PRSS3, Mesotrypsin)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

8B49

Host

Rat

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul sterile PBS to a concentration of 0.5 mg/ml.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes both pro and mature forms of human trypsin 3. Species sequence homology

Preparation and Storage

-20 degree C

Molecular Weight

32,529 Da

Chromosome Location

Chromosome: 9; NC_000009.11 (33750515..33799229). Location: 9p11.2

OMIM

613578

3D Structure

ModBase 3D Structure for P35030

Immunogen

NS0-derived recombinant human rhTrypsin-3.

Endotoxin Level

< 0.1 EU per 1ug of the antibody as determined by the LAL method. I

Storage and Stability:
Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile buffer. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hybridoma

Mouse myeloma cells were fused with B cells obtained from a rat immunized with purified, NS0-derived, recombinant human trypsin 3 encoded by the PRSS3 gene.

Product Categories/Family for anti-PRSS3 antibody

Antibodies; Abs to Enzymes

Applications Tested/Suitable for anti-PRSS3 antibody

Western Blot (WB), Neutralization

Application Notes for anti-PRSS3 antibody

Dilution: Neutralization:The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15ug/ml(IC 50) under conditions in which the enzyme was present at 0.03ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.

Other Notes

Small volumes of anti-PRSS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRSS3 purchase

MBS609301 is an antibody targeting PRSS3. Also known as Trypsin-3 (PRSS3, Mesotrypsin). Reactive in Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for PRSS3. It may not necessarily be applicable to this product.

NCBI GI #

308193325

NCBI GeneID

5646

NCBI Accession #

NP_001184027.1

UniProt Primary Accession #

P35030

UniProt Secondary Accession #

P15951; Q15665; Q5VXV0; Q9UQV3; A9Z1Y4

UniProt Related Accession #

P35030; Q6ISJ4; Q7Z5F4; Q8N2U3

GenBank Nucleotide #

NM_001197098.1

NCBI Official Full Name

trypsin-3 isoform 4 preproprotein

NCBI Official Synonym Full Names

protease, serine, 3

NCBI Official Symbol

PRSS3

NCBI Official Synonym Symbols

T9; MTG; TRY3; TRY4; PRSS4

UniProt Protein Name

Trypsin-3

UniProt Synonym Protein Names

Brain trypsinogen; Mesotrypsinogen; Serine protease 3; Serine protease 4; Trypsin III; Trypsin IV

Protein Family

Trypsin

UniProt Gene Name

PRSS3

UniProt Synonym Gene Names

PRSS4; TRY3; TRY4

UniProt Entry Name

TRY3_HUMAN

NCBI Protein Information

trypsin-3; trypsin 3; trypsin IV; mesotrypsin; trypsin III; trypsinogen 4; trypsinogen 5; trypsinogen IV; mesotrypsinogen; brain trypsinogen; serine protease 3; serine protease 4; OTTHUMP00000021238; OTTHUMP00000021239; pancreatic trypsinogen III; protease, serine, 4 (trypsin 4, brain)

NCBI Summary for PRSS3

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

UniProt Comments for PRSS3

trypsin 3: Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.4; Secreted; Calcium-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; digestion; innate immune response; endothelial cell migration; zymogen activation; proteolysis; water-soluble vitamin metabolic process

Research Articles on PRSS3

1. Report PRSS3/mesotrypsin upregulation in breast cancer cells and identify CD109 as the functional proteolytic target of mesotrypsin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PRSS3 antibody

Reference Product	PubMed Publications
APP antibody	>1 publications with PRSS3 and APP

Pathways associated with anti-PRSS3 antibody

Products by Pathway	Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram

Diseases associated with anti-PRSS3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>12 publications with PRSS3 and Neoplasms
Pancreatitis Antibodies	>7 publications with PRSS3 and Pancreatitis
Neoplasm Metastasis Antibodies	>3 publications with PRSS3 and Neoplasm Metastasis
Inflammation Antibodies	>3 publications with PRSS3 and Inflammation
Intestinal Diseases Antibodies	>3 publications with PRSS3 and Intestinal Diseases
Urologic Neoplasms Antibodies	>3 publications with PRSS3 and Urologic Neoplasms
Urinary Bladder Neoplasms Antibodies	>3 publications with PRSS3 and Urinary Bladder Neoplasms
Carcinoma, Squamous Cell Antibodies	>2 publications with PRSS3 and Carcinoma, Squamous Cell
Immune System Diseases Antibodies	>2 publications with PRSS3 and Immune System Diseases
Breast Neoplasms Antibodies	>2 publications with PRSS3 and Breast Neoplasms

Organs/Tissues associated with anti-PRSS3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>10 publications with PRSS3 and Brain
Pancreas Antibodies	>10 publications with PRSS3 and Pancreas
Intestine Antibodies	>3 publications with PRSS3 and Intestine
Eye Antibodies	>2 publications with PRSS3 and Eye
Skin Antibodies	>1 publications with PRSS3 and Skin