Rat Anti-IL1F10 Monoclonal Antibody – Cat# MBS609328

Interleukin 1, Family Member 10 (IL1F10) Antibody – Rat Monoclonal

Name: IL1F10 Antibody; Monoclonal; Rat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS609328
Price: 1025 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609328

Human IL-1F10/IL-1HY2 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse… More details

$1025 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 1, Family Member 10 (IL1F10), Monoclonal Antibody

Full Product Name

Interleukin 1, Family Member 10 (IL-1F10, Interleukin 1 Receptor Antagonist-like FIL1 theta, Interleukin 1 theta, IL-1 theta, FIL1 theta, Interleukin 1 HY2, IL-1HY2)

Product Gene Name

anti-IL1F10 antibody

Product Synonym Names

Anti -Interleukin 1, Family Member 10 (IL-1F10, Interleukin 1 Receptor Antagonist-like FIL1 theta, Interleukin 1 theta, IL-1 theta, FIL1 theta, Interleukin 1 HY2, IL-1HY2)

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

8H144

Host

Rat

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human IL-1F10. In direct ELISAs and Western Blots, this antibody shows approximately 50% crossreactivity with rmIL-1F10 and no crossreactivity with rhIL-1, rhIL-1, rhIL-1ra, rhIL-18, rhIL-1F5, rhIL-1F6, rhIL-1F7, rhIL-1F8, or rhIL-1F9.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

16,943 Da

Chromosome Location

Chromosome: 2; NC_000002.11 (113825547..113833427). Location: 2q13

3D Structure

ModBase 3D Structure for Q8WWZ1

Immunogen

E.coli-derived rhIL-1F10

Hybridoma

Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant human Interleukin 1 family member 10 (rhIL-1F10; aa 1-152; Accession # AAK68048).

Product Categories/Family for anti-IL1F10 antibody

Antibodies; Abs to Interleukin

Applications Tested/Suitable for anti-IL1F10 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-IL1F10 antibody

Western Blot, Direct ELISA
Dilution: Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human IL-1F10. Using a colorimetric detection system, the detection limit for rhIL-1F10 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human IL-1F10. The detection limit for rhIL-1F10 is approximately 2 ng/well.

Other Notes

Small volumes of anti-IL1F10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL1F10 purchase

MBS609328 is an antibody targeting IL1F10. Also known as Interleukin 1, Family Member 10 (IL-1F10, Interleukin 1 Receptor Antagonist-like FIL1 theta, Interleukin 1 theta, IL-1 theta, FIL1 theta, Interleukin 1 HY2, IL-1HY2). Reactive in Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for IL1F10. It may not necessarily be applicable to this product.

NCBI GI #

27894307

NCBI GeneID

84639

NCBI Accession #

NP_775184.1

UniProt Primary Accession #

Q8WWZ1

UniProt Secondary Accession #

Q53SR9; Q56AT8; Q7RTZ5; Q969H5; Q9BYX1

UniProt Related Accession #

Q8WWZ1

GenBank Nucleotide #

NM_173161.2

NCBI Official Full Name

interleukin-1 family member 10

NCBI Official Synonym Full Names

interleukin 1 family, member 10 (theta)

NCBI Official Symbol

IL1F10

NCBI Official Synonym Symbols

FKSG75; IL-1HY2; IL1-theta; MGC119831; MGC119832; MGC119833; FIL1-theta

UniProt Protein Name

Interleukin-1 family member 10

UniProt Synonym Protein Names

FIL1 theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 theta

Protein Family

Interleukin-1 family

UniProt Gene Name

IL1F10

UniProt Synonym Gene Names

FIL1T; IL1HY2

UniProt Entry Name

IL1FA_HUMAN

NCBI Protein Information

interleukin-1 family member 10; FIL1 theta; IL-1 theta; interleukin-1 HY2; OTTHUMP00000162018; OTTHUMP00000203696; interleukin-1 theta; IL-1F10 (canonical form IL-1F10a); interleukin-1 receptor antagonist FKSG75; interleukin-1 receptor antagonist-like FIL1 theta

NCBI Summary for IL1F10

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

UniProt Comments for IL1F10

IL1F10: Binds soluble IL-1 receptor type 1. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: extracellular space

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: cytokine and chemokine mediated signaling pathway

Research Articles on IL1F10

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-IL1F10 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with IL1F10 and Inflammation

Organs/Tissues associated with anti-IL1F10 antibody

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>1 publications with IL1F10 and Placenta